Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15 in FITC (MBL-K0227-4) is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 171

Product Specifications
Application FC
Reactivity Mouse
Conjugation BV421
Peptide Sequence FQPQNGQFI
Class Class I
Sequence Location 369-404
MHC Allele H-2Db


Documents & Links for Tetramer/BV421 - H-2 Db LCMV (FQPQNGQFI)
Datasheet Tetramer/BV421 - H-2 Db LCMV (FQPQNGQFI) Datasheet

Documents & Links for Tetramer/BV421 - H-2 Db LCMV (FQPQNGQFI)
Datasheet Tetramer/BV421 - H-2 Db LCMV (FQPQNGQFI) Datasheet