SARS-CoV-2 Spike glycoprotein Receptor-binding domain (Biotin Labeled)

Cosmo Bio LTD

Catalog No: HAK-SPD_BIO-1

When the new coronavirus infects a cell, the spike protein on the surface of the virus binds to ACE2 on the cell surface. Drugs that block this binding will prevent viral infection.

1. ACE2 extracellular domain
2. SARS-CoV-2 spike protein RBD/rabbit Fc tag
3. Biotinylated SARS-CoV-2 spike protein RBD and rabbit Fc tag
4. Neutralizing antibodies

We have also started selling a monoclonal antibody ( anti-SARS-CoV-2 spike protein monoclonal antibody (mouse)

) that is specific and has high affinity to the receptor binding domain of the spike protein S1 domain . This is a neutralizing antibody that inhibits the binding of the receptor binding domain to ACE2. 

• We also offer mutant strains. Please see here .

 

SARS-CoV-2 spike protein RBD/rabbit Fc tag

Biotinylated SARS-CoV-2 spike protein RBD and rabbit Fc tag

background

Angiotensin-converting enzyme 2 (ACE2), also known as ACE homolog, is an integral membrane protein that is highly homologous to ACE. ACE2 was known as one of the factors that work in the renin-angiotensin system, which is involved in blood pressure regulation. Recently, it has been revealed that when the novel coronavirus that causes COVID-19 infects human cells, it binds to ACE2 present in the cell membrane and is then taken into the cell, and ACE2 is also thought to be a receptor for the novel coronavirus. ⁽¹⁾ A highly sensitive binding assay is provided by immobilizing
human ACE2 protein with His tag (product number: HAK-ACE2_UL-1) on a 96-well plate and adding SARS-CoV-2 spike protein RBD with rabbit Fc tag (product number: HAK-SPD_UL-1) or biotinylated SARS-CoV-2 spike protein RBD with rabbit Fc tag (product number: HAK-SPD_bio-1), which is a fusion protein in which the receptor binding domain of the viral protein is bound to rabbit Fc, and detecting with an HRP-labeled secondary antibody or streptavidin.

Protocol for binding experiment between SARS-CoV-2 spike protein and ACE2

Detailed protocols for binding assays and competitive inhibitor screening are available. You can download the PDF
here .

 

 

 

Biotinylated SARS-CoV-2 spike protein RBD and rabbit Fc tag

Product Information

Fig. 6 SDS-PAGE

Protein Structure
Biotinylated SARS-CoV-2 spike protein RBD with rabbit Fc tag (HAK-SPD_bio-1) is produced by attaching a rabbit IgG1 Fc tag to the C-terminus of Arg319-Phe541 of the SARS-CoV-2 Spike glycoprotein receptor-binding domain, expressing it in HEK293 cells, purifying it using a protein A column, and biotinylated it.
purity
>95% (SDS-PAGE)
composition
SARS-CoV-2 spike protein RBD/rabbit Fc tag 1mg/mL, 0.1M-PBS(pH7.2~7.4)
keep
Avoid repeated freeze-thaw cycles. Upon receipt, store product at -70°C or colder. Aliquoting is recommended prior to use.
SDS-PAGE
500ng of this product before biotinylation was electrophoresed on a 4-20% gradient gel and stained with CBB (Figure 6).

 

Usage Example

1. Binding assay
• 29-21000ng/mL of biotinylated SARS-CoV-2 spike protein RBD and rabbit Fc tag were bound to 1µg/mL of solid-phased human ACE2 protein (100µL/well) and detected with HRP-labeled streptavidin. The linear range of the calibration curve was 29-798ng/mL (Figure 7).

Figure 7 Binding assay