When the new coronavirus infects a cell, the spike protein on the surface of the virus binds to ACE2 on the cell surface. Drugs that block this binding will prevent viral infection.
- ACE2 extracellular domain
- SARS-CoV-2 spike protein RBD/rabbit Fc tag
- Biotinylated SARS-CoV-2 spike protein RBD and rabbit Fc tag
- Neutralizing antibodies
We have also started selling a monoclonal antibody ( anti-SARS-CoV-2 spike protein monoclonal antibody (mouse)
) that is specific and has high affinity to the receptor binding domain of the spike protein S1 domain . This is a neutralizing antibody that inhibits the binding of the receptor binding domain to ACE2.
- We also offer mutant strains. Please see here .
SARS-CoV-2 spike protein RBD/rabbit Fc tag
Biotinylated SARS-CoV-2 spike protein RBD and rabbit Fc tag
background
Angiotensin-converting enzyme 2 (ACE2), also known as ACE homolog, is an integral membrane protein that is highly homologous to ACE. ACE2 was known as one of the factors that work in the renin-angiotensin system, which is involved in blood pressure regulation. Recently, it has been revealed that when the novel coronavirus that causes COVID-19 infects human cells, it binds to ACE2 present in the cell membrane and is then taken into the cell, and ACE2 is also thought to be a receptor for the novel coronavirus. (1) A highly sensitive binding assay is provided by immobilizing
human ACE2 protein with His tag (product number: HAK-ACE2_UL-1) on a 96-well plate and adding SARS-CoV-2 spike protein RBD with rabbit Fc tag (product number: HAK-SPD_UL-1) or biotinylated SARS-CoV-2 spike protein RBD with rabbit Fc tag (product number: HAK-SPD_bio-1), which is a fusion protein in which the receptor binding domain of the viral protein is bound to rabbit Fc, and detecting with an HRP-labeled secondary antibody or streptavidin.
Protocol for binding experiment between SARS-CoV-2 spike protein and ACE2
Detailed protocols for binding assays and competitive inhibitor screening are available. You can download the PDF
here .
Product Information
Fig. 6 SDS-PAGE
| Protein Structure |
|---|
| Biotinylated SARS-CoV-2 spike protein RBD with rabbit Fc tag (HAK-SPD_bio-1) is produced by attaching a rabbit IgG1 Fc tag to the C-terminus of Arg319-Phe541 of the SARS-CoV-2 Spike glycoprotein receptor-binding domain, expressing it in HEK293 cells, purifying it using a protein A column, and biotinylated it. |
| purity |
| >95% (SDS-PAGE) |
| composition |
| SARS-CoV-2 spike protein RBD/rabbit Fc tag 1mg/mL, 0.1M-PBS(pH7.2~7.4) |
| keep |
| Avoid repeated freeze-thaw cycles. Upon receipt, store product at -70°C or colder. Aliquoting is recommended prior to use. |
| SDS-PAGE |
| 500ng of this product before biotinylation was electrophoresed on a 4-20% gradient gel and stained with CBB (Figure 6). |
Usage Example
- Binding assay
- 29-21000ng/mL of biotinylated SARS-CoV-2 spike protein RBD and rabbit Fc tag were bound to 1µg/mL of solid-phased human ACE2 protein (100µL/well) and detected with HRP-labeled streptavidin. The linear range of the calibration curve was 29-798ng/mL (Figure 7).
Figure 7 Binding assay
| Product Specifications | |
| Conjugation | Biotin |
| Documents & Links for SARS-CoV-2 Spike glycoprotein Receptor-binding domain (Biotin Labeled) | |
| Datasheet | SARS-CoV-2 Spike glycoprotein Receptor-binding domain (Biotin Labeled) Datasheet |
| Documents & Links for SARS-CoV-2 Spike glycoprotein Receptor-binding domain (Biotin Labeled) | |
| Datasheet | SARS-CoV-2 Spike glycoprotein Receptor-binding domain (Biotin Labeled) Datasheet |