SARS-CoV-2 Spike Glycoprotein ELISA Kit

Cosmo Bio

SKU: HAK-HELSRBD-1

Anti-SARS-CoV-2 spike protein antibody is immobilized on the plate. When the sample is added, the SARS-CoV-2 spike protein in the sample is trapped. After washing, the trapped protein is reacted with an HRP-labeled anti-SARS-CoV-2 spike protein antibody, and after substrate addition, the color development by HRP is read and quantified with a plate reader.

Features:

• Measures SARS-CoV-2 spike protein in samples such as serum and plasma.
• No special equipment required, measurement is possible with a normal plate reader.
• SARS-CoV-2 spike protein can be measured sensitively and specifically by sandwich ELISA method using two types of antibodies against RBD in the SARS-CoV-2 S1 domain.

The COVID-19 pandemic, which originated in China in 2019, has spread worldwide, and as of the end of July 2021, 197.19 million people worldwide have been infected and 4.21 million people have died. SARS-CoV-2, identified as the causative virus of Covid-19, is a type of coronavirus with a gene encoding an RNA sequence of approximately 30,000 base pairs in length. The spike protein encoded by the S gene in the viral genome is a membrane protein expressed on the surface of the virus. The spike protein consists of two domains, S1 and S2.
When SARS-CoV-2 infects a cell, it has been revealed that the S1 domain of the spike protein first binds to angiotensin-converting enzyme 2 (ACE2) on the cell surface, and then the virus is taken into the cell. ACE2 is thought to be the receptor for SARS-CoV-2. Furthermore, structural analysis of the S1 domain of the spike protein has identified a narrow region (receptor binding domain, RBD) that binds to ACE2 (1).
This product can measure SARS-CoV-2 spike protein in samples by sandwich ELISA using two types of neutralizing antibodies that are specific and have high affinity for the RBD of the spike protein S1 domain.

Features

• It is possible to measure the SARS-CoV-2 spike protein in samples such as serum and plasma.
• No special equipment is required; measurements can be performed with a standard plate reader.
• A sandwich ELISA using two antibodies against the RBD of the SARS-CoV-2 S1 domain is used to measure SARS-CoV-2 spike protein with high sensitivity and specificity.
• The antibodies used in this ELISA react strongly with the Wuhan variant as well as all alpha, beta, gamma, and delta variants of the virus (paper in preparation).

Kit Principle

Anti-SARS-CoV-2 spike protein antibody is immobilized on the plate, and when a sample is added, the SARS-CoV-2 spike protein in the sample is trapped. After washing, the trapped protein is reacted with HRP-labeled anti-SARS-CoV-2 spike protein antibody, and after adding the substrate, the color development by HRP is read and quantified with a plate reader.

Measurement example

Exosomes (15.625, 31.25, 62.5, 125, 250, 500, 1000ng/mL) purified by ultracentrifugation from the culture supernatant of Expi293 cells expressing SARS-Cov-2 spike protein (S1, S2 domains) were added to each well in 100mµL portions and measured. The exosome concentration (ng/mL) and absorbance (OD450) are shown in Figure 1.

Figure 1. Measurement of exosomes expressing SARS-Cov-2 spike protein (S1, S2 domains)