R-spondin 1 (roof plate-specific spondin 1), also known as Christine 3, is one of the R-spondin family members that regulates to the activation of canonical WNT/β-catenin signaling.
In the absence of R-spondin, Frizzled (Fz) is endocytosed by being ubiquitinated by the binding of the ubiquitin ligase RNF43/ZNRF3, Fz on the cell membrane decreases, and Wnt signals are not activated enough.
Conversely, in the presence of R-spondin, RNF43/ZNRF3 forms trimers with LGR and R-spondin and is endocytosed, making it unable to ubiquitinate Fz. As a result, Fz on the cell membrane increases, and Wnt signals are strongly activated.
This product is a bioactive domain consisting of 21 to 146 amino acids which contain two adjacent cysteine-rich furin-like domains (Fu1 and Fu2). Fu1 binds to the extracellular domain of ZNRF3*1 and RNF43*2, and Fu2 binds to the extracellular domain of LGR4/5/6.
Recombinant protein of human R-spondin 1 (21-146 aa) is expressed in E. coli.
*1 zinc and ring finger 3, *2 ring finger protein 43, *3 leucine-rich repeat-containing G-protein coupled receptors 4/5/6
Organoid culture is typically utilized niche factors that act on Wnt, EGF, BMP and Notch signals that control stem cell differentiation. Among these signals, R-spondin 1 contributes to the activation of Wnt signals.
Notes When culturing organoids, stem cells, or other tissues, if you are to use this product in combination with other factor or factors (hereunder factors), a third party may have a patent on the use or other application of the factors concerned. Regarding to this product, we do not offer any non-infringement warranty when used or otherwise applied in combination with other factors. Therefore, if you intend to use this product in combination with other factors, please check with your organization’s division responsible for intellectual property rights or your research agency before using this product.
