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PUREfrex® 2.0 reconstituted coupled transcription/translation system is a new and improved version of PUREfrex® 1.0 that debuted in 2011. By improving the purification process for components of PUREfrex® 2.0RNase, β-galactosidase and LPS contamination has been reduced, the latter to around 0.1EU/µL of reaction mixture. This, together with a newly optimized composition has boosted protein yield 2-10 times compared to PUREfrex® 1.0. Further, all proteinaceous components of PUREfrex® 2.0 are free of fusion tags, allowing users the freedom to incorporate any chosen tag for protein purification/detection.
For proteins whose functional conformation requires the assistance of molecular chaperones or the creation of disulfide bonds, we offer supplemental reagents that work in conjunction with PUREfrex® 2.0.
Supplements – Molecular Chaperones
These are supplements for PUREfrex® series reconstituted coupled transcription/translation systems to promote production and solubility of aggregation-prone proteins.
Supplements – Forming disulfide bonds
These are supplements for PUREfrex® series reconstituted coupled transcription/translation systems to promote synthesis of functional proteins containing disulfide bonds.
| Documents & Links for PUREfrex 2.0 | |
| Manual | PUREfrex® 2.0 Instruction Manual |
| Datasheet | PUREfrex 2.0 Product Insert (Size: 250ul Cat.No.:GFK-PF201-0.25-EX) |
| Flyer | PUREfrex 2.0 Flyer |
| Datasheet | PUREfrex 2.0 Product Insert (Size: 250ul x 5 Cat.No.:GFK-PF201-0.25-5-EX) |
| Datasheet | PUREfrex 2.0 Product Insert (Size: 2ml Cat.No.:GFK-PF201-2ML-EX) |
| Datasheet | PUREfrex 2.0 Product Insert (Size: 2ml x 5 Cat.No.:GFK-PF201-10ML-EX) |
| Documents & Links for PUREfrex 2.0 | |
| Manual | PUREfrex® 2.0 Instruction Manual |
| Datasheet | PUREfrex 2.0 Product Insert (Size: 250ul Cat.No.:GFK-PF201-0.25-EX) |
| Flyer | PUREfrex 2.0 Flyer |
| Datasheet | PUREfrex 2.0 Product Insert (Size: 250ul x 5 Cat.No.:GFK-PF201-0.25-5-EX) |
| Datasheet | PUREfrex 2.0 Product Insert (Size: 2ml Cat.No.:GFK-PF201-2ML-EX) |
| Datasheet | PUREfrex 2.0 Product Insert (Size: 2ml x 5 Cat.No.:GFK-PF201-10ML-EX) |
| Citations for PUREfrex 2.0 – 17 Found |
| Curli synthesis and biofilm formation in enteric bacteria are controlled by a dynamic small RNA module made up of a pseudoknot assisted by an RNA chaperone. Nucleic Acids Res. 2014 Apr;42(7):4682-96. doi: 10.1093/nar/gku098. Epub 2014 Feb 1. PubMed |
| Cell-free synthesis of the torque-generating membrane proteins, PomA and PomB, of the Na+-driven flagellar motor in Vibrio alginolyticus. J Biochem. 2008 Nov;144(5):635-42. doi: 10.1093/jb/mvn110. Epub 2008 Sep 6. PubMed |
| RNA, but not protein partners, is directly responsible for translational silencing by a bacterial Hfq-binding small RNA. Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10332-7. doi: 10.1073/pnas.0803106105. Epub 2008 Jul 23. PubMed |
| YaeJ is a novel ribosome-associated protein in Escherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes. Nucleic Acids Res. 2011 Mar;39(5):1739-48. doi: 10.1093/nar/gkq1097. Epub 2010 Nov 3. PubMed |
| Salmonella enterica serotype Typhimurium DT104 ArtA-dependent modification of pertussis toxin-sensitive G proteins in the presence of [32P]NAD. Microbiology (Reading). 2009 Nov;155(Pt 11):3710-3718. doi: 10.1099/mic.0.028399-0. Epub 2009 Aug 20. PubMed |
| SmpB triggers GTP hydrolysis of elongation factor Tu on ribosomes by compensating for the lack of codon-anticodon interaction during trans-translation initiation. J Biol Chem. 2006 Jun 9;281(23):15987-96. doi: 10.1074/jbc.M512165200. Epub 2006 Apr 6. PubMed |
| Roles of Tom70 in import of presequence-containing mitochondrial proteins. J Biol Chem. 2009 Nov 13;284(46):31635-46. doi: 10.1074/jbc.M109.041756. Epub 2009 Sep 18. PubMed |
| In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme. Nucleic Acids Res. 2010 Jul;38(13):e141. doi: 10.1093/nar/gkq377. Epub 2010 May 10. PubMed |
| A small RNA regulates multiple ABC transporter mRNAs by targeting C/A-rich elements inside and upstream of ribosome-binding sites. Genes Dev. 2007 Nov 1;21(21):2804-17. doi: 10.1101/gad.447207. PubMed |
| A multiprotein binding interface in an intrinsically disordered region of the tumor suppressor protein interferon regulatory factor-1. J Biol Chem. 2011 Apr 22;286(16):14291-303. doi: 10.1074/jbc.M110.204602. Epub 2011 Jan 18. PubMed |
| Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import. J Biol Chem. 2008 Feb 15;283(7):3799-807. doi: 10.1074/jbc.M708339200. Epub 2007 Dec 6. PubMed |
| Cortisol is involved in temperature-dependent sex determination in the Japanese flounder. Endocrinology. 2010 Aug;151(8):3900-8. doi: 10.1210/en.2010-0228. Epub 2010 Jun 9. PubMed |
| Role of antibiotic ligand in nascent peptide-dependent ribosome stalling. Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10496-501. doi: 10.1073/pnas.1103474108. Epub 2011 Jun 13. PubMed |
| In vitro methods for peptide display and their applications. Brief Funct Genomics. 2011 May;10(3):125-34. doi: 10.1093/bfgp/elr010. PubMed |
| Functional role played by the glycosylphosphatidylinositol anchor glycan of CD48 in interleukin-18-induced interferon-gamma production. J Biol Chem. 2005 May 6;280(18):18056-62. doi: 10.1074/jbc.M413297200. Epub 2005 Mar 10. PubMed |
| Involvement of Hu and heterogeneous nuclear ribonucleoprotein K in neuronal differentiation through p21 mRNA post-transcriptional regulation. J Biol Chem. 2005 Apr 1;280(13):12690-9. doi: 10.1074/jbc.M411119200. Epub 2005 Jan 25. PubMed |
| Curli synthesis and biofilm formation in enteric bacteria are controlled by a dynamic small RNA module made up of a pseudoknot assisted by an RNA chaperone. Nucleic Acids Res. 2014 Apr;42(7):4682-96. doi: 10.1093/nar/gku098. Epub 2014 Feb 1. PubMed |