PUREfrex 2.0

Cosmo Bio LTD

Catalog No: GFK-PF201-0.1-EX

Explore all PUREfrex® products and accessories from our PUREfrex® homepage

PUREfrex Products: Quick Access

Kits	Supplements
PUREfrex 2.0 PUREfrex 2.1 PUREfrex 1.0	DnaK Mix GroE Mix DsbC Set PDI Set EF-P

PUREfrex^® 2.0 reconstituted coupled transcription/translation system is a new and improved version of PUREfrex^® 1.0 that debuted in 2011. By improving the purification process for components of PUREfrex^® 2.0RNase, β-galactosidase and LPS contamination has been reduced, the latter to around 0.1EU/µL of reaction mixture. This, together with a newly optimized composition has boosted protein yield 2-10 times compared to PUREfrex^® 1.0. Further, all proteinaceous components of PUREfrex^® 2.0 are free of fusion tags, allowing users the freedom to incorporate any chosen tag for protein purification/detection.

New tester format available! GFK-PF201-0.1-EX: 100uL mini kit has been introduced so that you can evaluate if your protein can be synthesized with PureFrex, allowing you the opportunity to troubleshoot and optimize your template with a lower volume. Common primers are included so you do not need to acquire them separately.

For proteins whose functional conformation requires the assistance of molecular chaperones or the creation of disulfide bonds, we offer supplemental reagents that work in conjunction with PUREfrex^® 2.0.

Supplements – Molecular Chaperones

These are supplements for PUREfrex^® series reconstituted coupled transcription/translation systems to promote production and solubility of aggregation-prone proteins.

• DnaK Mix (#PF003-0.5-EX)
• GroE Mix (#PF004-0.5-EX)

Supplements – Forming disulfide bonds

These are supplements for PUREfrex^® series reconstituted coupled transcription/translation systems to promote synthesis of functional proteins containing disulfide bonds.

• DsbC Set (#PF005-0.5-EX)
• PDI Set (#PF006-0.5-EX)

Citations for PUREfrex 2.0 – 17 Found

Bordeau, Valérie; Felden, Brice. Curli synthesis and biofilm formation in enteric bacteria are controlled by a dynamic small RNA module made up of a pseudoknot assisted by an RNA chaperone. Nucleic Acids Research. 2014;42(7):4682-96. 

Terashima, Hiroyuki; Abe-Yoshizumi, Rei; Kojima, Seiji; Homma, Michio. Cell-free synthesis of the torque-generating membrane proteins, PomA and PomB, of the Na+-driven flagellar motor in Vibrio alginolyticus. Journal Of Biochemistry. 2008;144(5):635-42. 

Maki, Kimika; Uno, Kanako; Morita, Teppei; Aiba, Hiroji. RNA, but not protein partners, is directly responsible for translational silencing by a bacterial Hfq-binding small RNA. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2008;105(30):10332-7. 

Handa, Yoshihiro; Inaho, Noriyuki; Nameki, Nobukazu. YaeJ is a novel ribosome-associated protein in Escherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes. Nucleic Acids Research. 2011;39(5):1739-48. 

Uchida, Ikuo; Ishihara, Ryoko; Tanaka, Kiyoshi; Hata, Eiji; Makino, Sou-Ichi; Kanno, Toru; Hatama, Shinichi; Kishima, Masato; Akiba, Masato; Watanabe, Atsushi; Kubota, Takayuki. Salmonella enterica serotype Typhimurium DT104 ArtA-dependent modification of pertussis toxin-sensitive G proteins in the presence of [32P]NAD. Microbiology (Reading, England). 2009;155(Pt 11):3710-3718. 

Shimizu, Yoshihiro; Ueda, Takuya. SmpB triggers GTP hydrolysis of elongation factor Tu on ribosomes by compensating for the lack of codon-anticodon interaction during trans-translation initiation. The Journal Of Biological Chemistry. 2006;281(23):15987-96. 

Yamamoto, Hayashi; Fukui, Kenji; Takahashi, Hisashi; Kitamura, Shingo; Shiota, Takuya; Terao, Kayoko; Uchida, Mayumi; Esaki, Masatoshi; Nishikawa, Shuh-ichi; Yoshihisa, Tohru; Yamano, Koji; Endo, Toshiya. Roles of Tom70 in import of presequence-containing mitochondrial proteins. The Journal Of Biological Chemistry. 2009;284(46):31635-46. 

Asahara, Haruichi; Chong, Shaorong. In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme. Nucleic Acids Research. 2010;38(13):e141. 

Sharma, Cynthia M; Darfeuille, Fabien; Plantinga, Titia H; Vogel, Jörg. A small RNA regulates multiple ABC transporter mRNAs by targeting C/A-rich elements inside and upstream of ribosome-binding sites. Genes & Development. 2007;21(21):2804-17. 

Narayan, Vikram; Halada, Petr; Hernychová, Lenka; Chong, Yuh Ping; Žáková, Jitka; Hupp, Ted R; Vojtesek, Borivoj; Ball, Kathryn L. A multiprotein binding interface in an intrinsically disordered region of the tumor suppressor protein interferon regulatory factor-1. The Journal Of Biological Chemistry. 2011;286(16):14291-303. 

Yamano, Koji; Yatsukawa, Yoh-Ichi; Esaki, Masatoshi; Hobbs, Alyson E Aiken; Jensen, Robert E; Endo, Toshiya. Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import. The Journal Of Biological Chemistry. 2008;283(7):3799-807. 

Yamaguchi, Toshiya; Yoshinaga, Norifumi; Yazawa, Takashi; Gen, Koichiro; Kitano, Takeshi. Cortisol is involved in temperature-dependent sex determination in the Japanese flounder. Endocrinology. 2010;151(8):3900-8. 

Vázquez-Laslop, Nora; Klepacki, Dorota; Mulhearn, Debbie C; Ramu, Haripriya; Krasnykh, Olga; Franzblau, Scott; Mankin, Alexander S. Role of antibiotic ligand in nascent peptide-dependent ribosome stalling. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2011;108(26):10496-501. 

Ullman, Christopher G; Frigotto, Laura; Cooley, R Neil. In vitro methods for peptide display and their applications. Briefings In Functional Genomics. 2011;10(3):125-34. 

Fukushima, Keiko; Ikehara, Yukio; Yamashita, Katsuko. Functional role played by the glycosylphosphatidylinositol anchor glycan of CD48 in interleukin-18-induced interferon-gamma production. The Journal Of Biological Chemistry. 2005;280(18):18056-62. 

Yano, Masato; Okano, Hirotaka J; Okano, Hideyuki. Involvement of Hu and heterogeneous nuclear ribonucleoprotein K in neuronal differentiation through p21 mRNA post-transcriptional regulation. The Journal Of Biological Chemistry. 2005;280(13):12690-9. 

Bordeau, Valérie; Felden, Brice. Curli synthesis and biofilm formation in enteric bacteria are controlled by a dynamic small RNA module made up of a pseudoknot assisted by an RNA chaperone. Nucleic Acids Research. 2014;42(7):4682-96.