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Innovative immunoassay to assess semen quality in horse
The proAKAP4 ELISA kit is the first immunoassay that validates objectively semen quality by quantifying a protein biomarker named proAKAP4 in samples of horse semen. This ELISA Kit is the 1st functional testto evaluate sperm quality.
The dosage of the proAKAP4 biomarker with the ELISA Kit will bring you:
• Tool for the selection of the breeding males
• Qualification of each breeding male
• Qualification of male ejaculates before and after cryopreservation
The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation.
Studies are currently conducted together with the "European Horse Center of Mont Le Soie Linalux" (Belgium) and the research teams of SPQI - 4BioDx.
Technical data
- 40 samples can be run in duplicate
- Working with fresh ejaculate or with frozen semen
- Easy to use immunoassay / Manual and automated assay
- Robust and reproductible method
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Reagents provided: The complete proAKAP4 ELISA kit contains all reagents and buffers required for the quantification of in horse semen.
- One Microplate pre-coated with the Capture Antibody
- One vial of Standard
- One vial of Detection Antibody
- One bottle of the Enzyme Reagent
- One bottle of 10X Wash Buffer
- One bottle of the Sample Diluant
- One bottle of the Acid Stop Solution
- Three ELISA plate sealers
| Documents & Links for proAKAP4 4MID Kit (ELISA, Horse) | |
| Manual | proAKAP4 4MID Kit (ELISA, Horse) Manual |
| Vendor Page | proAKAP4 4MID Kit (ELISA, Horse) at 4BioDx |
| Documents & Links for proAKAP4 4MID Kit (ELISA, Horse) | |
| Manual | proAKAP4 4MID Kit (ELISA, Horse) Manual |
| Vendor Page | proAKAP4 4MID Kit (ELISA, Horse) |
| Citations for proAKAP4 4MID Kit (ELISA, Horse) – 3 Found |
| First results about ProAKAP4 concentration in stallion semen after cryopreservation in two different freezing media. Cryobiology. 2021 Oct;102:133-135. doi: 10.1016/j.cryobiol.2021.07.009. Epub 2021 Jul 21. PubMed |
| The Concentration of ProAKAP4 and Other Indicators of Cryopotential of Spermatozoa Cryopreserved in Extender with Holothuroidea Extract Addition. Animals (Basel). 2022 Feb 20;12(4):521. doi: 10.3390/ani12040521. PubMed |
| Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: Promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. 2019 Jun;131:52-60. doi: 10.1016/j.theriogenology.2019.03.011. Epub 2019 Mar 21. PubMed |