Mitophagy detection vectors are designed for the co-expression of MT-mKeima-Red. (CoralHue® Mitochondria-targeted monomeric Keima-Red) gene and mouse Parkin (mPark2) gene from the internal ribosome entry site (IRES), as part of a bicistronic transcript in mammalian cells. Keima-Red has been cloned from Montipara sp. A monomeric version of CoralHue® Keima-Red (mKeima-Red) displays a bimodal excitation spectrum with peaks at 440 and 586nm in neutral and acidic solutions, respectively, and an emission maximum at 620nm. Mitochondrial targeting of mKeima-Red is achieved by introducing a mitochondrial signal sequence at the N-terminus of mKeima-Red.