Leptin ELISA Kit (mouse)

Cosmo Bio LTD

Catalog No: YII-YK052-EX

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This ELISA kit is used for quantitative determination of mouse leptin in its serum samples. The kit is characterized by its sensitive quantification and high specificity. In addition, it is not influenced by other constituents in samples. Standard antigen, mouse leptin, in this kit is a recombinant product.

This ELISA kit is highly specific to mouse leptin. It shows 33.8 % crossreactivity to rat leptin and no crossreactivity to human leptin. Supplementary: Sandwich

Applicable Sample: Serum, Plasma

Other: Leptin, which is a product of ob gene, is a protein consisting of 146 amino acids and it is secreted from white adipose tissue. It is known that leptin acts on hypothalamus to decrease food intake and reduce body weight, body fat, blood sugar and blood insulin in a healthy and an ob/ob mice. Further, gene expression of neuropeptide Y (NPY) is suppressed by this protein. Recently, radioimmunoassay for leptin determination inhuman plasma has become available and leptin level in human patient group with obesity was found to increase in comparison with that in normal group. The plasma level well correlated with body fat and these observations showed clearly that leptin concentration in human plasma reflects tissue fat weight. The measurement of leptin in plasma or serum may be a good index of obesity. Although mouse leptin shows a high homology (96%) with rat leptin in amino acid sequences, it is observed that substitution of quite a number of residues occurs between human and mouse leptin. These findings have urged us to develop highly sensitive immunoassay system specific to rat or mouse leptin.