L-glutamate oxidase (EC 1.4.3.11) is an enzyme that catalyzes oxidative deamination of the α-amino group of L-glutamate to 2-ketoglutarate, with concomitant reduction of molecular oxygen and water to ammonia and hydrogen peroxide. L-glutamate oxidase is a member of the general oxidoreductase family, which catalyzes the CH-OH group of the substrate and uses NAD+ or NADP+ as the cofactor.
The systematic name of this enzyme class is L-glutamate:oxygen oxidoreductase (deaminating), which is also called glutamate (acceptor) dehydrogenase, glutamate oxidase, glutamic acid oxidase, glutamic dehydrogenase (acceptor) or L-glutamic acid oxidase. L-glutamate, the substrate of the reaction catalyzed by L-glutamate oxidase, has a flavor-enhancing property that creates the sensation of “umami”, and the monosodium salt of L-glutamate is widely used as a seasoning for cooking and as a food additive.
Moreover, L-glutamate is also the principal excitatory neurotransmitter in the brain. Furthermore, the excessive release of this amino acid may play a pivotal role in the neuronal death associated with different neurological disorders. Therefore, L-glutamate and L-glutamate oxidase play important roles in the fields of food production and clinical biochemistry.
Specifications |
|
Appearance | Lyophilized powder |
Contents | 25 units/vial |
Source | E. coli |
Molecular weight | 140 kDa |
Subunit structure | α2β2γ2(α: 43,000, β: 17,000, γ: 10,000) |
Thermo stability | stable below 60°C (30 min at pH 6) |
pH stability | pH 4-10 (60 min at 50ºC) |
Optimum pH | pH 7-9 |
Km | 0.2 mM |
Substrate specificity | L-Glu 100%; L-Gln 0%; L-Asp 0%; D-Glu 0%; 0% for 16 other L-amino acids |
Unit definition | One unit equals the amount of enzyme that liberates 1 micromole 2-ketoglutarate per minute with 3-methyl-2- benzothiazolone hydrazone hydrochloride as substrate at 30°C, pH 7.4 (Soda K, et al. 1968). |
Reaction | L-Glutamate + O2 + H2O ---> alpha-Ketoglutarate + NH3 + H2O2 |
Cofactor | FAD (2 mol per mol of enzyme) |
Storage and Stability
Lyophilized powder may be stored desiccated at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Prepare fresh before use.
References
1.Arima, J. et al., J. Biochem. 134, 805-812(2003)
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Documents & Links for L-Glutamate Oxidase 25 | |
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Citations for L-Glutamate Oxidase 25 – 4 Found |
Enhanced Cognition and Hypoglutamatergic Signaling in a Growth Hormone Receptor Knockout Mouse Model of Successful Aging. J Gerontol A Biol Sci Med Sci. 2017 Mar 1;72(3):329-337. doi: 10.1093/gerona/glw088. PubMed |
Development of a novel micro biosensor for in vivo monitoring of glutamate release in the brain. Biosens Bioelectron. 2019 Apr 1;130:103-109. doi: 10.1016/j.bios.2019.01.049. Epub 2019 Jan 30. PubMed |
Diet-induced insulin resistance elevates hippocampal glutamate as well as VGLUT1 and GFAP expression in AβPP/PS1 mice. J Neurochem. 2019 Jan;148(2):219-237. doi: 10.1111/jnc.14634. Epub 2019 Jan 3. PubMed |
3D printed microfluidic device for online detection of neurochemical changes with high temporal resolution in human brain microdialysate. Lab Chip. 2019 Jun 7;19(11):2038-2048. doi: 10.1039/c9lc00044e. Epub 2019 May 16. PubMed |