L-Glutamate Oxidase 25

Cosmo Bio LTD

Catalog No: YMS-80049

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L-glutamate oxidase (EC 1.4.3.11) is an enzyme that catalyzes oxidative deamination of the α-amino group of L-glutamate to 2-ketoglutarate, with concomitant reduction of molecular oxygen and water to ammonia and hydrogen peroxide. L-glutamate oxidase is a member of the general oxidoreductase family, which catalyzes the CH-OH group of the substrate and uses NAD+ or NADP+ as the cofactor.

The systematic name of this enzyme class is L-glutamate:oxygen oxidoreductase (deaminating), which is also called glutamate (acceptor) dehydrogenase, glutamate oxidase, glutamic acid oxidase, glutamic dehydrogenase (acceptor) or L-glutamic acid oxidase. L-glutamate, the substrate of the reaction catalyzed by L-glutamate oxidase, has a flavor-enhancing property that creates the sensation of “umami”, and the monosodium salt of L-glutamate is widely used as a seasoning for cooking and as a food additive.

Moreover, L-glutamate is also the principal excitatory neurotransmitter in the brain. Furthermore, the excessive release of this amino acid may play a pivotal role in the neuronal death associated with different neurological disorders. Therefore, L-glutamate and L-glutamate oxidase play important roles in the fields of food production and clinical biochemistry.

 

Specifications
Appearance	Lyophilized powder
Contents	25 units/vial
Source	E. coli
Molecular weight	140 kDa
Subunit structure	α2β2γ2(α: 43,000, β: 17,000, γ: 10,000)
Thermo stability	stable below 60°C (30 min at pH 6)
pH stability	pH 4-10 (60 min at 50ºC)
Optimum pH	pH 7-9
Km	0.2 mM
Substrate specificity	L-Glu 100%; L-Gln 0%; L-Asp 0%; D-Glu 0%; 0% for 16 other L-amino acids
Unit definition	One unit equals the amount of enzyme that liberates 1 micromole 2-ketoglutarate per minute with 3-methyl-2- benzothiazolone hydrazone hydrochloride as substrate at 30°C, pH 7.4 (Soda K, et al. 1968).
Reaction	L-Glutamate + O2 + H2O ---> alpha-Ketoglutarate + NH3 + H2O2
Cofactor	FAD (2 mol per mol of enzyme)

Storage and Stability

Lyophilized powder may be stored desiccated at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Prepare fresh before use.

References

1.Arima, J. et al., J. Biochem. 134, 805-812(2003)

Citations for L-Glutamate Oxidase 25 – 5 Found

Hascup, Kevin N; Lynn, Mary K; Fitzgerald, Patrick J; Randall, Shari; Kopchick, John J; Boger, Heather A; Bartke, Andrzej; Hascup, Erin R. Enhanced Cognition and Hypoglutamatergic Signaling in a Growth Hormone Receptor Knockout Mouse Model of Successful Aging. The Journals Of Gerontology. Series A, Biological Sciences And Medical Sciences. 2017;72(3):329-337. 

Ganesana, Mallikarjunarao; Trikantzopoulos, Elefterios; Maniar, Yash; Lee, Scott T; Venton, B Jill. Development of a novel micro biosensor for in vivo monitoring of glutamate release in the brain. Biosensors & Bioelectronics. 2019;130( 30731343):103-109. 

Hascup, Erin R; Broderick, Sarah O; Russell, Mary K; Fang, Yimin; Bartke, Andrzej; Boger, Heather A; Hascup, Kevin N. Diet-induced insulin resistance elevates hippocampal glutamate as well as VGLUT1 and GFAP expression in AβPP/PS1 mice. Journal Of Neurochemistry. 2019;148(2):219-237. 

Samper, Isabelle C; Gowers, Sally A N; Rogers, Michelle L; Murray, De-Shaine R K; Jewell, Sharon L; Pahl, Clemens; Strong, Anthony J; Boutelle, Martyn G. 3D printed microfluidic device for online detection of neurochemical changes with high temporal resolution in human brain microdialysate. Lab On A Chip. 2019;19(11):2038-2048. 

Robbins, Elaine M; Wong, Benjamin; Pwint, May Yoon; Salavatian, Siamak; Mahajan, Aman; Cui, Xinyan Tracy. Improving Sensitivity and Longevity of In Vivo Glutamate Sensors with Electrodeposited NanoPt. Acs Applied Materials & Interfaces. 2024; 39078097( 39078097)