KBM Neural Stem Cell (Serum-free basal medium)

Cosmo Bio LTD

Catalog No: KJN-16050100

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KBM Neural Stem Cell Series＋ KBM XB2

Capable of both undifferentiated proliferation and inducing differentiation of NSCs.
(Serum-free medium for Neural stem cells)

KBM Neural Stem Cell

• This medium is specialized for the neurosphere assay which is widely applied research and development regarding neural stem cells (NSCs) and neural cells. By adding KBM Neural Stem Cell Supplement to this medium and proceeding suspension culture, it is capable of culturing NSCs as neurospheres. Cultured neurospheres can produce new neurospheres by dispersing cells and re-culturing. It is also capable of inducing differentiation of NSCs into neural cells by adherent cell cultures of neurospheres, without addition of supplement.
  
  
• A kit based on a test developed by the Japan Fruit Juice Association, detects contamination with Alicyclobacillus acidoterrestris and related Thermo-acidophilic Bacilli (TAB) by their production of guaiacol.

Product Features

【KBM Neural Stem Cell + Supplement】

• Serum-free and chemically-defined
• Capable of both undifferentiated proliferation and inducing differentiation of NSCs.
• Confirmed the capability to culture NSCs derived human iPS cells.

【KBM XB2】

• Chemically-defined spplement(50x concentration)
• Contains retinoic acid(vitaminA)

Culture Examples

(a)PDT variation at passages of NSCs	(b)Total number of NSCs at passages	(c)Picture of cultured neurospheres
(d)Picture of differentiated neurons	(e)Picture of cultured neurospheres

 

	Culture Conditions (a,b,c)	Differentiation Induction Assay(d)	Culture Condition(e)
Culture medium	KBM Neural Stem Cell＋KBM Neural Stem Cell Supplement	KBM Neural Stem Cell	KBM Neural stem cell + KBM XB2
Cell culture vessel	6 well cell culture plate（corning 3471）	Cell culture cover glass Poly-L-ornithine coating	Cell culture cover glass Poly-L-ornithine coating
Protocols	Cells were seeded at 1×105 cells/mL, and passaged every 5 days.	Neurospheres were seeded and cultured for 5 days. Neurospheres were seeded and cultured for 5 days
Antibodies		Green : anti-β tubulin ⅢBlue : anti-GFAPRed : anti-CNPase	Green：TuJ-1

 Product Lineup

Catalog Number	Product name	Size
KJN-16050100	KBM Neural Stem Cell	500mL
KJN-16050300	KBM Neural Stem Cell Supplement	1mL
KJN-16050400	KBM XB2	10mL

Citations for KBM Neural Stem Cell (Serum-free basal medium) – 3 Found

Nakamura, Ryota; Nonaka, Risa; Oyama, Genko; Jo, Takayuki; Kamo, Hikaru; Nuermaimaiti, Maierdanjiang; Akamatsu, Wado; Ishikawa, Kei-Ichi; Hattori, Nobutaka. A defined method for differentiating human iPSCs into midbrain dopaminergic progenitors that safely restore motor deficits in Parkinson's disease. Frontiers In Neuroscience. 17( 37502682):1202027. 

Ago, Kentaro; Nagoshi, Narihito; Imaizumi, Kent; Kitagawa, Takahiro; Kawai, Momotaro; Kajikawa, Keita; Shibata, Reo; Kamata, Yasuhiro; Kojima, Kota; Shinozaki, Munehisa; Kondo, Takahiro; Iwano, Satoshi; Miyawaki, Atsushi; Ohtsuka, Masanari; Bito, Haruhiko; Kobayashi, Kenta; Shibata, Shinsuke; Shindo, Tomoko; Kohyama, Jun; Matsumoto, Morio; Nakamura, Masaya; Okano, Hideyuki. A non-invasive system to monitor in vivo neural graft activity after spinal cord injury. Communications Biology. 2022;5(1):803. 

Kawai, Momotaro; Imaizumi, Kent; Ishikawa, Mitsuru; Shibata, Shinsuke; Shinozaki, Munehisa; Shibata, Takahiro; Hashimoto, Shogo; Kitagawa, Takahiro; Ago, Kentaro; Kajikawa, Keita; Shibata, Reo; Kamata, Yasuhiro; Ushiba, Junichi; Koga, Keisuke; Furue, Hidemasa; Matsumoto, Morio; Nakamura, Masaya; Nagoshi, Narihito; Okano, Hideyuki. Long-term selective stimulation of transplanted neural stem/progenitor cells for spinal cord injury improves locomotor function. Cell Reports. 2021;37(8):110019.