KBM 551 (Medium for Activation and Expansion Culture of Human T Cells)
This medium was developed to support the growth of T cells activated with immobilized anti-CD3 antibody.
The addition of interleukin-2 is necessary for the culture.
This product is a medium suitable for both activated and expanded culture of human peripheral blood T cells stimulated with solid phase anti-CD3 antibody.
Add 5 to 10% serum (plasma) at the start of activation culture.
Addition of serum (plasma) may be omitted during the expansion culture.
It does not contain human interleukin-2 (IL-2), and the addition is required at the time of use.
KBM 550, which contains IL-2, is also available.
You can choose either according to your purpose.
Features
●This media is prepared from pharmaceutical grade water and other high purity pharmaceutical grade components.
●This medium does not contain proteins except for human serum albumin (pharmaceutical grade), recombinant human insulin.
●Capable of minimize the variation of pH by enhanced buffer capacity.
●Kanamycin sulfate is contained as antibiotic.
●The medium has good keeping quality.
Usage Examples
1. This medium is optimized for activation and expansion culture of human T cells activated with immobilized anti-CD3 antibody.
2. Add 5-10% autoserum or autoplasma at the onset of culture and necessary quantity of human interleukin-2.
3. Upon cell proliferation, add autoserum (autoplasma) and medium culture which includes interleukin-2, scale up the culture. There would be a case to skip adding autoserum(autoplasma) this time.
Osmolality: 295 ± 10 mOsm/kg H2O (determined by freezing point depression osmometry)
pH: 7.2 ± 0.2 (determined using a glass electrode)
Sterilization: Negative (Membrane filtration)
Mycoplasma Test: Negative (PCR detection)
Endotoxin Test: Less than 0.3 EU/mL (Determined by Limulus amebocyte lysate assay)
Notice: This product is only for research use, and not for human or animal therapeutic use.
Performance evaluation of KBM551:
KBM 551 Vessel: 12-well plate on which anti-human-CD3 antibody is immobilized.
Cell: Normal human peripheral blood monocytes
Cytokine: Recombinant human interleukin-2 (175 IU/mL) was added.
Plasma: 5% inactivated autoplasma was added at the onset of culture.
Proliferative test: The culture was started at a seeding density of 6 × 105 cells/mL, under static conditions at 37℃, 5% CO2. Upon cell proliferation, the culture was diluted by adding each medium containing the cytokine without plasma.
Analysis: At day 14, the cytotoxicity to K562 cells were measured with TeraScan that is a microfluorocytometer developed for measuring cytotoxicity.
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