Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 67
| Product Specifications | |
| Application | FC |
| Reactivity | mouse |
| Conjugation | BV421 |
| Peptide Sequence | RAHYNIVTF |
| Citations for iTAg Tetramer/BV421 - H-2 Db HPV 16 E7 (RAHYNIVTF) – 1 Found |
| Quayle, Steven N; Girgis, Natasha; Thapa, Dharma R; Merazga, Zohra; Kemp, Melissa M; Histed, Alex; Zhao, Fan; Moreta, Miguel; Ruthardt, Paige; Hulot, Sandrine; Nelson, Alyssa; Kraemer, Lauren D; Beal, Dominic R; Witt, Luke; Ryabin, Jessica; Soriano, Jonathan; Haydock, Mark; Spaulding, Emily; Ross, John F; Kiener, Peter A; Almo, Steven; Chaparro, Rodolfo; Seidel, Ronald; Suri, Anish; Cemerski, Saso; Pienta, Kenneth J; Simcox, Mary Ellen. CUE-101, a Novel E7-pHLA-IL2-Fc Fusion Protein, Enhances Tumor Antigen-Specific T-Cell Activation for the Treatment of HPV16-Driven Malignancies. Clinical Cancer Research : An Official Journal Of The American Association For Cancer Research. 2020;26(8):1953-1964. PubMed |