Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 57
| Product Specifications | |
| Application | FC |
| Reactivity | Mouse |
| Conjugation | APC |
| Peptide Sequence | KAVYNFATC |
| Citations for iTAg Tetramer/APC - H-2 Db LCMV (KAVYNFATC) – 4 Found |
| Gil, M Pilar; Salomon, Rachelle; Louten, Jennifer; Biron, Christine A. Modulation of STAT1 protein levels: a mechanism shaping CD8 T-cell responses in vivo. Blood. 2006;107(3):987-93. PubMed |
| Jung, Andreas; Kato, Hiroki; Kumagai, Yutaro; Kumar, Himanshu; Kawai, Taro; Takeuchi, Osamu; Akira, Shizuo. Lymphocytoid choriomeningitis virus activates plasmacytoid dendritic cells and induces a cytotoxic T-cell response via MyD88. Journal Of Virology. 2008;82(1):196-206. PubMed |
| Kawagoe, Tatsukata; Sato, Shintaro; Jung, Andreas; Yamamoto, Masahiro; Matsui, Kosuke; Kato, Hiroki; Uematsu, Satoshi; Takeuchi, Osamu; Akira, Shizuo. Essential role of IRAK-4 protein and its kinase activity in Toll-like receptor-mediated immune responses but not in TCR signaling. The Journal Of Experimental Medicine. 2007;204(5):1013-24. PubMed |
| Takagi, Akira; Matsui, Masanori; Ohno, Satoshi; Duan, Hongying; Moriya, Osamu; Kobayashi, Nobuharu; Oda, Hiroshi; Mori, Masahito; Kobayashi, Akiharu; Taneichi, Maiko; Uchida, Tetsuya; Akatsuka, Toshitaka. Highly efficient antiviral CD8+ T-cell induction by peptides coupled to the surfaces of liposomes. Clinical And Vaccine Immunology : Cvi. 2009;16(10):1383-92. PubMed |