Human iPSC Complete Reprogramming Kit

Cellular Engineering Technologies

SKU: CET-MR1003-K

Human iPSC Complete Reprogramming Kit

Our Human iPSC Complete Reprogramming Kit is specifically designed for the safe and efficient reprogramming of primary cells, enabling the generation of induced pluripotent stem cells (iPSCs) from various starting cell types. Moreover, this kit is versatile and can be used with primary fibroblasts or peripheral blood mononuclear cells (PBMCs), including lymphocytes, monocytes, and dendritic cells. Whether for adherent growth or suspension-based applications, our reprogramming protocol adapts to either traditional electroporation or chemically mediated transfection, providing flexibility to accommodate customer preferences in transduction methods.

The kit utilizes our proprietary episomal reprogramming method, which requires only a single transfection step for efficient cell reprogramming. As a result, it includes a carefully selected combination of episomal DNA vectors and small molecule inhibitors, enabling robust reprogramming while preserving pluripotency markers. For optimal results, we recommend targeting low passage, mitotically active cells, as they yield the best reprogramming efficiency.

Included Components

• Human iPSC Reprogramming Base Media (250 mL)
• Human iPSC Reprogramming Supplement (495 µL)
• Human iPSC Episomal DNA Mix (Cat. CET-MR1004)
• Human iPSC Growth Media Kit (Cat. CET-MR1001-K)
  • Human iPSC Growth Base Media (500 mL)
  • Human iPSC Growth Media Supplement (7 mL)

Key Features & Quality Control

• Efficient Reprogramming: Our single-step transfection protocol ensures high reprogramming efficiency without the use of viral vectors or oncogenic transcription factors like C-myc, L-myc, and LIN28.
• Versatile Use: Suitable for reprogramming many cell types including fibroblasts and PBMCs from a variety of human tissues.
• Maintains Pluripotency: Supports the maintenance of pluripotent stem cell markers throughout the reprogramming process and beyond.
• Optimized Protocol: Compatible with both adherent and suspension-based applications, providing flexibility for diverse research needs.
• Comprehensive Kit: Includes all necessary reagents for both reprogramming and growth of iPSCs, streamlining the process for researchers.

Applications of iPSCs

• Stem Cell Research: Generate iPSCs for disease modeling, regenerative medicine, and cell-based therapies.
• Gene Editing: Create iPSC lines for CRISPR/Cas9-mediated gene editing and genetic disease studies.
• Tissue Engineering: Create and then differentiate iPSCs into various cell types for regenerative medicine and tissue engineering applications.
• Drug Discovery & Toxicology: Use iPSCs as models for drug screening and toxicity testing in human-relevant systems.

This Human iPS Cell Complete Reprogramming Kit is an essential tool for researchers working in stem cell biology, regenerative medicine, and translational research. Order today and streamline your reprogramming process with our complete solution!