iPS Cell Episomal Reprogramming Kit

Cellular Engineering Technologies

SKU: CET-MR1004-K

Human iPSC Episomal DNA Reprogramming Kit

Our proprietary Human iPSC Episomal DNA Reprogramming plasmid mix is specifically designed to reprogram any starting cell type into induced pluripotent stem cells (iPSCs) with high efficiency. Moreover, this ready-to-use iPSC reprogramming kit includes individual reprogramming factors (RFs) such as Oct-4, Sox-2, and Klf-4, each contained in EBNA-1 vectors with a CMV promoter and poly A sequences for optimized expression. Episomal reprogramming ensures safe and effective reprogramming while avoiding the oncogenic risks associated with c-Myc, l-Myc, and Lin28 transcription factors.

In addition to the core RFs, this mix includes a p53 anti-sense element to protect cells from apoptosis during early reprogramming stages and a Red Fluorescent Protein (RFP) Reporter for tracking transfection efficiency and episomal DNA silencing.

Our  reprogramming methodology offers several key advantages over traditional reprogramming methods, including:

1. Single transfection step for efficient reprogramming.
2. Clearance of episomal DNA during cell division, unlike the persistent viral DNA from the Sendai Virus method.
3. No viral protein expression as seen with other viral-based methods.
4. Cost-effective solution with fewer transfection steps, providing significant savings compared to other reprogramming techniques.

This iPSC Reprogramming kit enables consistent and reproducible results, ideal for research in cell therapy, regenerative medicine, and genetic reprogramming.

Each vial contains 50µg of cryopreserved episomal reprogramming DNA for easy storage and reliable use.

Key Features & Benefits:

• High reprogramming efficiency with Oct-4, Sox-2, and Klf-4 factors.
• Safe, non-viral approach with no oncogenic c-Myc or Lin28.
• Single transfection step for cost and time savings.
• Cryopreserved for long-term storage.
• Ideal for use in iPSC differentiation, cell-based therapies, and stem cell research.

Applications:

• Reprogramming of any starter cell types, including:
  • Fibroblasts
  • CD34+ cord blood-derived cells
  • PBMCs