Hyaluronan Binding Protein (HABP), recombinant Versican G1 domain (Biotin Conj.)

Cosmo Bio LTD

Catalog No: HKD-BC41

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hyaluronan purification with affinity column Hyaluronan binding protein rHABP is produced by expression induced culturing in the presence of IPTG using E.coli BL21(DE3)RIL transfected with human versican G1-domain expression vector pRK172VG1. As compared with animal-derived HABP,the high biological safety is confirmed. rHAB doesn't bind to other Glycosaminoglycan and DNA and specifically detects the hyaluronan (HA).Biotin is attached to rHAB to make the biotin-HABP.

Purpose of Use

For detection of the hyaluronan (HA)
Histochemistry and Cytochemistry
Detection of membrane-transferred hyaluronan
ELISA

Description

Versican G1 domain bind specifically to hyaluronan and doesn't bind to other glycosaminoglycan DNA.
Versican G1 domain can use probe for hyaluronan detection.

References

•  Tengblad A. et al., Biochem J. Nov1;199(2):297-305 (1981)
•  Tengblad A. et al., Biochem J. Jan 1;185(1):101-5 (1980)
◊  Invest. Ophthalmol. Vis. Sci.,?Aug 2011; 52: 6511 - 6521

Citations for Hyaluronan Binding Protein (HABP), recombinant Versican G1 domain (Biotin Conj.) – 3 Found

Clark, Simon J; Keenan, Tiarnan D L; Fielder, Helen L; Collinson, Lisa J; Holley, Rebecca J; Merry, Catherine L R; van Kuppevelt, Toin H; Day, Anthony J; Bishop, Paul N. Mapping the differential distribution of glycosaminoglycans in the adult human retina, choroid, and sclera. Investigative Ophthalmology & Visual Science. 2011;52(9):6511-21. 

Tengblad, A. A comparative study of the binding of cartilage link protein and the hyaluronate-binding region of the cartilage proteoglycan to hyaluronate-substituted Sepharose gel. The Biochemical Journal. 1981;199(2):297-305. 

Tengblad, A. Quantitative analysis of hyaluronate in nanogram amounts. The Biochemical Journal. 1980;185(1):101-5.