Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 175
| Product Specifications | |
| Application | FC |
| Reactivity | Mouse |
| Conjugation | PE |
| Peptide Sequence | HA |
| Citations for H-2Kd Influenza HA Tetramer-IYSTVASSL-PE – 3 Found |
| Huang, Xiaopei; Yang, Yiping. The fate of effector CD8 T cells in vivo is controlled by the duration of antigen stimulation. Immunology. 2006;118(3):361-71. PubMed |
| Kayamuro, Hiroyuki; Yoshioka, Yasuo; Abe, Yasuhiro; Arita, Shuhei; Katayama, Kazufumi; Nomura, Tetsuya; Yoshikawa, Tomoaki; Kubota-Koketsu, Ritsuko; Ikuta, Kazuyoshi; Okamoto, Shigefumi; Mori, Yasuko; Kunisawa, Jun; Kiyono, Hiroshi; Itoh, Norio; Nagano, Kazuya; Kamada, Haruhiko; Tsutsumi, Yasuo; Tsunoda, Shin-ichi. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. Journal Of Virology. 2010;84(24):12703-12. PubMed |
| Sasaki, Eita; Asanuma, Hideki; Momose, Haruka; Furuhata, Keiko; Mizukami, Takuo; Hamaguchi, Isao. Immunogenicity and Toxicity of Different Adjuvants Can Be Characterized by Profiling Lung Biomarker Genes After Nasal Immunization. Frontiers In Immunology. 11:2171. PubMed - Comments: Used in: FCM |