Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 176
| Product Specifications | |
| Application | FC |
| Reactivity | Mouse |
| Conjugation | APC |
| Peptide Sequence | HA |
| Citations for H-2Kd Influenza HA Tetramer-IYSTVASSL-APC – 1 Found |
| Kayamuro, Hiroyuki; Yoshioka, Yasuo; Abe, Yasuhiro; Arita, Shuhei; Katayama, Kazufumi; Nomura, Tetsuya; Yoshikawa, Tomoaki; Kubota-Koketsu, Ritsuko; Ikuta, Kazuyoshi; Okamoto, Shigefumi; Mori, Yasuko; Kunisawa, Jun; Kiyono, Hiroshi; Itoh, Norio; Nagano, Kazuya; Kamada, Haruhiko; Tsutsumi, Yasuo; Tsunoda, Shin-ichi. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. Journal Of Virology. 2010;84(24):12703-12. PubMed |
