Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 147
| Product Specifications | |
| Application | FC |
| Reactivity | Mouse |
| Conjugation | APC |
| Citations for H-2Kb Negative Tetramer-SIYRYYGL-APC – 3 Found |
| Wakita, Daiko; Chamoto, Kenji; Zhang, Yue; Narita, Yoshinori; Noguchi, Daisuke; Ohnishi, Hideaki; Iguchi, Takeshi; Sakai, Tomoaki; Ikeda, Hiroaki; Nishimura, Takashi. An indispensable role of type-1 IFNs for inducing CTL-mediated complete eradication of established tumor tissue by CpG-liposome co-encapsulated with model tumor antigen. International Immunology. 2006;18(3):425-34. PubMed |
| Chamoto, Kenji; Wakita, Daiko; Narita, Yoshinori; Zhang, Yue; Noguchi, Daisuke; Ohnishi, Hideaki; Iguchi, Takeshi; Sakai, Tomoaki; Ikeda, Hiroaki; Nishimura, Takashi. An essential role of antigen-presenting cell/T-helper type 1 cell-cell interactions in draining lymph node during complete eradication of class II-negative tumor tissue by T-helper type 1 cell therapy. Cancer Research. 2006;66(3):1809-17. PubMed |
| Asano, Jumpei; Tada, Hiroyuki; Onai, Nobuyuki; Sato, Taku; Horie, Yasuo; Fujimoto, Yukari; Fukase, Koichi; Suzuki, Akira; Mak, Tak W; Ohteki, Toshiaki. Nucleotide oligomerization binding domain-like receptor signaling enhances dendritic cell-mediated cross-priming in vivo. Journal Of Immunology (Baltimore, Md. : 1950). 2010;184(2):736-45. PubMed |