Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 157
| Product Specifications | |
| Application | FC |
| Reactivity | Mouse |
| Conjugation | PE |
| Peptide Sequence | KSPWFTTL |
| Citations for H-2Kb MuLV p15E Tetramer-KSPWFTTL-PE – 2 Found |
| Kudo-Saito, Chie; Ishida, Akiko; Shouya, Yuji; Teramoto, Koji; Igarashi, Tomoyuki; Kon, Ryoko; Saito, Kenji; Awada, Chihiro; Ogiwara, Yamato; Toyoura, Masayoshi. Blocking the FSTL1-DIP2A Axis Improves Anti-tumor Immunity. Cell Reports. 2018;24(7):1790-1801. PubMed |
| Sivick, Kelsey E; Desbien, Anthony L; Glickman, Laura Hix; Reiner, Gabrielle L; Corrales, Leticia; Surh, Natalie H; Hudson, Thomas E; Vu, Uyen T; Francica, Brian J; Banda, Tamara; Katibah, George E; Kanne, David B; Leong, Justin J; Metchette, Ken; Bruml, Jacob R; Ndubaku, Chudi O; McKenna, Jeffrey M; Feng, Yan; Zheng, Lianxing; Bender, Steven L; Cho, Charles Y; Leong, Meredith L; van Elsas, Andrea; Dubensky, Thomas W Jr; McWhirter, Sarah M. Magnitude of Therapeutic STING Activation Determines CD8(+) T Cell-Mediated Anti-tumor Immunity. Cell Reports. 2018;25(11):3074-3085.e5. PubMed |