Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 181
| Product Specifications | |
| Application | FC |
| Reactivity | Mouse |
| Conjugation | PE |
| Peptide Sequence | Glycoprotein B |
| Citations for H-2Kb HSV-1 gB Tetramer-SSIEFARL-PE – 3 Found |
| Fukaya, Tomohiro; Murakami, Ryuichi; Takagi, Hideaki; Sato, Kaori; Sato, Yumiko; Otsuka, Haruna; Ohno, Michiko; Hijikata, Atsushi; Ohara, Osamu; Hikida, Masaki; Malissen, Bernard; Sato, Katsuaki. Conditional ablation of CD205+ conventional dendritic cells impacts the regulation of T-cell immunity and homeostasis in vivo. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2012;109(28):11288-93. PubMed |
| Aoki, Rui; Kawamura, Tatsuyoshi; Goshima, Fumi; Ogawa, Youichi; Nakae, Susumu; Nakao, Atsuhito; Moriishi, Kohji; Nishiyama, Yukihiro; Shimada, Shinji. Mast cells play a key role in host defense against herpes simplex virus infection through TNF-α and IL-6 production. The Journal Of Investigative Dermatology. 2013;133(9):2170-9. PubMed |
| Imai, Takahiko; Koyanagi, Naoto; Ogawa, Ryo; Shindo, Keiko; Suenaga, Tadahiro; Sato, Ayuko; Arii, Jun; Kato, Akihisa; Kiyono, Hiroshi; Arase, Hisashi; Kawaguchi, Yasushi. Us3 kinase encoded by herpes simplex virus 1 mediates downregulation of cell surface major histocompatibility complex class I and evasion of CD8+ T cells. Plos One. 8(8):e72050. PubMed |
