H-2Db gp100 Tetramer-EGSRNQDWL-PE

MBL Life Sciences

SKU: MBL-TS-M546-1

Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 257

Product Specifications

• Application: FC
• Reactivity: Mouse
• Conjugation: PE
• Peptide Sequence: EGSRNQDWL
• Class: Class I
• Sequence Location: 25-33
• MHC Allele: H-2D^b

Citations for H-2Db gp100 Tetramer-EGSRNQDWL-PE – 1 Found

Hanson, Melissa C; Crespo, Monica P; Abraham, Wuhbet; Moynihan, Kelly D; Szeto, Gregory L; Chen, Stephanie H; Melo, Mariane B; Mueller, Stefanie; Irvine, Darrell J. Nanoparticulate STING agonists are potent lymph node-targeted vaccine adjuvants. The Journal Of Clinical Investigation. 2015;125(6):2532-46.