GroE Mix

Cosmo Bio

Catalog No: GFK-PF004-0.5-EX

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Kits	Supplements
PUREfrex 2.0 PUREfrex 2.1 PUREfrex 1.0	DnaK Mix GroE Mix DsbC Set PDI Set EF-P

GroE Mix is a supplement for the PUREfrex^® series reconstituted coupled transcription/translation systems, designed to promote proper folding and solubility of proteins that require molecular chaperones for proper protein folding. GroE Mix comprises an optimized ratio of highly purified E.coli-derived GroEL (Hsp60) and GroES.

Chaperone Supplement

DnaK Mix comprises highly purified and ratio-optimized E. coli DnaK, DnaJ and GrpE. DnaK, known as Hsp70, has ATPase activity and is stimulated by co-chaperones DnaJ and GrpE. DnaJ facilitates DnaK ATPase activity and binds to hydrophobic regions of synthesized proteins. GrpE stimulates ADP/ATP exchange of DnaK. DnaK Mix works well with PUREfrex^®series and DsbC Set.

Chaperone Supplement

GroE Mix comprises highly purified and ratio-optimized E. coli GroEL (known as Hsp60) and GroES (works in conjunction with GroEL). 

Reagent	Qty	Description	Storage
GroE Mix	12.5 ul	20µM GroEL, 14-mer form, and 40µM GroES, 7-mer form, from E.coli in 30% glycerol buffer (No tags)	-80 C
Dilution Buffer	500 ul	30% glycerol buffer	-20 C

Reagent	Qty	Description	Storage
DnaK Mix	25 ul	100µM DnaK, 20µM DnaJ and 20µM GrpE from E.coli in 30% glycerol buffer (No tags)	-80 C
Dilution Buffer	500 ul	30% glycerol buffer	-20 C