EzWay Direct ApoE Genotyping Kit

LABISKOMA

Catalog No: KBT-K0568500

Please note that Koma Biotech items have a $300 minimum. Please contact us if you have any questions.

Features:


• ApoE genotyping directly from blood.

• One-step Multiplex PCR system with PrimerMix

• ApoE primer mixture for E2 (Cys112/Cys158), E3 (Cys112/Arg158) and E4 (Arg112/Arg158)

• Fast and simple method for genotyping without DNA purification

• Minimize the risk of DNA loss or contamination

• Optimized MasterMix type containing Taq enzyme, dNTP, Direct PCR Buffer, MgCl2, Red dye and additives

• Useful detection of high cholesterol levels, cardiovascular disease or Alzheimer's disease  


Applications: ApoE genotyping  

Description

Human Apolipoprotein E (ApoE) is a 34 kDa glycoprotein that plays a central role in lipid metabolism and transportation. The ApoE gene is polymorphic with three common alleles, designated E2, E3, and E4. These genes encode three ApoE protein isoforms, E2 (Cys112/Cys158), E3 (Cys112/Arg158) and E4 (Arg112/Arg158), that differ by cysteine-arginine interchanges at sites 112 and 158 in the polypeptide chain. The common ApoE isoforms exhibit variations in structure and function and are involved in several pathological processes. For instance, familial type III hyperlipoproteinemia is associated with the ApoE E2/E2 pheno/genotype, while the ApoE E4/E4 pheno/genotype is associated with high cholesterol levels, coronary artery disease, and Alzheimer’s disease.

EzWay Direct ApoE Genotyping Kit is useful for simple ApoE genotyping from whole blood without DNA extraction by using direct PCR technology. Also, the primer mixture of ApoE genes enables to perform one-step Multiplex PCR. Specification • ApoE genotyping directly from blood
• One-step Multiplex PCR system with PrimerMix
• ApoE primer mixture for E2 (Cys112/Cys158), E3 (Cys112/Arg158) and E4 (Arg112/Arg158)
• Fast and simple method for genotyping without DNA purification
• Minimize the risk of DNA loss or contamination
• Optimized MasterMix type containing Taq enzyme, dNTP, Direct PCR Buffer, MgCl2, Red dye and additives
• Useful detection of high cholesterol levels, cardiovascular disease or Alzheimer's disease

Components

• 2X EzWay™ Direct Hot Taq PCR MasterMix
• 2.5X ApoE PrimerMix
• Size Marker
• Positive Control

Citations

The Influence of Baseline Alzheimer's Disease Severity on Cognitive Decline and CSF Biomarkers in the NILVAD Trial
Laila Abdullah, Fiona Crawford1,2, Magda Tsolaki, Anne Börjesson-Hanson4, Marcel Olde Rikkert, Florence Pasquier, Anders Wallin, Sean Kennelly, Ghania Ait-Ghezala, Daniel Paris, Suzanne Hendrix, Kaj Blennow, Brian Lawlor and Michael Mullan
Front. Neurol., 06 March 2020

Exploratory analyses suggest less cognitive decline with nilvadipine treatment in very mild Alzheimer’s disease subjects
L Abdullah, F Crawford, H Langlois, M Tsolaki, A Börjesson-Hanson, M Olde Rikkert, F Pasquier, A Wallin, S Kennelly, S Hendrix, K Blennow, B Lawlor, M Mullan
bioRxiv, 2019

Enhanced capillary electrophoretic screening of Alzheimer based on direct apolipoprotein E genotyping and one-step multiplex PCR
N Woo, SK Kim, Y Sun, SH Kang
Journal of Chromatography B, 2018

Genetics and Other Risk Factors for Past Concussions in Active-Duty Soldiers
MN Dretsch, N Silverberg, AJ Gardner, WJ Panenka, T Emmerich, G Crynen, G Ait-Ghezala, H Chaytow, V Mathura, FC Crawford, GL Iverson
Journal of Neurotrauma VOL. 34, NO. 4, 2017

Brain-derived neurotropic factor polymorphisms, traumatic stress, mild traumatic brain injury, and combat exposure contribute to postdeployment traumatic stress
MN Dretsch, K Williams, T Emmerich, G Crynen, G Ait-Ghezala, H Chaytow, V Mathura, FC Crawford, GL Iverson
Brain and Behavior, 2016; 6(1), e00392