Endo-CC N180H (Mutant)

Cosmo Bio LTD

Catalog No: FSP-171832

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Product Name: Endo-CC N180H
Package:300munits
Source:Endo-CC N180H recombinant from Coprinopsis cinerea is expressed in E.coli as a fusion to His tag.
Unit Definition:One unit is defined as the amount of enzyme that produce 1μmol SG-GlcNAc-pNP from pNP-GlcNAc per minute at 30˚C, pH7.5.

Background
Endo-β-N-acetylglucosaminidase (ENGase) catalyzes hydrolysis of N-linked oligosaccharides.
In addition, some ENGases have transglycosylation activity, and can transfer sugar chains to sugar or glycoconjugate as acceptor.

Endo-CC is an ENGase from the basidiomycete fungus Coprinopsis cinerea, and catalyzes hydrolysis of N-linked oligosaccharides, especially high-mannose type and biantennary complex type.
Endo-CC N180H is Endo-CC mutant, and can transfer sugar chains for example SG-Oxazoline (product number:171810) as donor to GlcNAc as acceptor.

Reference

• 1) Eshima Y, Higuchi Y, Kinoshita T, Nakakita S, Takegawa K:
  Transglycosylation activity of glycosynthase mutants of endo-β-N-acetylglucosaminidase from Coprinopsis cinerea.
  PloS one 10, e0132859 (2015)
• 2) Higuchi Y, Eshima Y, Huang Y, Kinoshita T, Sumiyoshi W, Nakakita S, Takegawa K:
  Highly efficient transglycosylation of sialo-complex-type oligosaccharide using Coprinopsis cinerea endoglycosidase and sugar oxazoline.
  Biotechnol. Lett. 39, 157-162 (2017)
• 3) Manabe S, Yamaguchi Y, Abe J, Matsumoto K, Ito Y:
  Acceptor range of endo-β-N-acetylglucosaminidase mutant endo-CC N180H:from monosaccharide to antibody.
  R Soc Open Sci, 5 (5) , 171521 2018 16 eCollection May (2018)