DsbC Set

Cosmo Bio

SKU: GFK-PF005-0.5-EX

Redox Environment Supplements Promote Disulfide Bond Formation
Formation of disulfide bonds is important for folding and stability of secretory proteins such as enzymes or antibodies. Disulfide bonds are usually formed by oxidation of sulfhydryl groups (SH-) of adjacent cysteine residues. Therefore, disulfide bond formation efficiency depends on redox state. Additionally, disulfide bond isomerase which can catalyze the exchange of disulfide bridges may be required for correct cysteine pairing.

Redox Supplement

DsbC Set comprises highly purified E. coli DsbC (a disulfide bond isomerase) which can catalyze disulfide bridge exchange, and GSSG (oxidized Glutathione) to enforce an oxidized environment.

 

• Reagent	Qty	Description	Storage
  GSSG	12.5 ul	Oxidized glutathione (60 mM)	-20 C
  DSBC	25 ul	E.coli DsbC (320 µM *2) (No tags)	-80 C
  Dilution Buffer	500ul	30% glycerol buffer	-20 C

Redox Supplement

PDI Set comprises oxidized glutathione (GSSG), human PDI (protein disulfide isomerase) and human Ero1α (ER oxidoreductin-1 to reoxidize PDI).

• Reagent	Qty	Description	Storage
  GSSG	12.5 ul	Oxidized glutathione (60 mM)	-20 C
  PDI	25 ul	Human protein disulfide isomerase with no tags (200 µM)	-80 C
  Ero1a	25 ul	Human ER oxidoreductin-1 to reoxidize PDI with no tags (5 µM)	-80 C
  Dilution Buffer	500 ul	30% glycerol buffer	-20 C