Cryopreservation Media

Cellular Engineering Technologies

SKU: CET-MR1014

Cryopreservation Media

Cryopreservation Media is a highly versatile, high-performance formulation designed to support the long-term storage of both adherent and suspension cells. This specialized media ensures maximum post-thaw viability and recovery across a wide range of cell types, including hematopoietic stem cells, mesenchymal stem cells, amniotic epithelial stem cells, cancer cells, endothelial cells, fibroblasts, and immune cells. It contains 10% DMSO, a cryoprotectant that helps prevent ice crystal formation, minimizing cellular damage during the freezing and thawing process.

Engineered for broad compatibility, our Cryopreservation Media is optimized for use with all CET.bio adherent and non-adherent cell lines, which are available separately. When used according to protocol, this media enables indefinite cryogenic storage at temperatures ranging from -80°C to -196°C. While cells can be kept at -80°C for short-term storage, optimal long-term preservation requires storage in the vapor phase of liquid nitrogen or in an ultra-low freezer capable of reaching temperatures below -150°C.

Usage & Handling of Cryopreservation Media:

To ensure the best results, Cryopreservation Media should be stored at -20°C and thawed properly before use. Extended exposure to room temperature should be avoided, as temperature fluctuations may impact performance. Before adding to any cell culture, the media should be equilibrated in a water bath set at 37°C. Following proper cryopreservation protocols will help maintain cell integrity and viability upon thawing.

Recommended Uses:

• Compatible with a broad range of cell types, including stem cells, immune cells, fibroblasts, and more [i] [ii] [iii].
• Designed for use with CET.bio’s complete catalog of adherent and suspension cell lines.

Shipping & Storage:

• Shipped with dry ice to maintain stability during transport.
• Store at -20°C for optimal performance and longevity.