CELLiST™ FEED Media
CELLiST™ FEED Media is a highly concentrated feed medium designed for achieving optimal culture performance in CHO cell lines. CELLiST™ FEED Media will ensure optimal culture growth and productivity, as well as protein quality, to satisfy your process goals. CELLiST™ FEED Media could be used in combination with any media, although designed and optimized for use with CELLiST™ BASAL Media.
Features
- Chemically formulated and free from animal-derived ingredients
- Contains high-quality amino acids based on the Ajinomoto's long-standing amino acid research capabilities and expertise
- Suitable for most CHO cell lines including CHO-K1, CHO-GS, CHO-S, CHO-DG44 and CHO-DXB11
- Packaging form can be adjusted according to customer’s requirements
Specifications
| Model No. | FEED2 |
| TYPE | Powder |
| Concentration |
110.0g / L |
| With Additives | None |
| Storage Condition | 2℃ to 8℃, dark and dry |
| Shelf Life | 36 months |
| Item Description | Powder, chemically defined and animal derived component free medium, rehydrate to 1 L |
Performance
CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 / BASAL10 with FEED2
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against five popular commercial media brands in fed-batch culture. 6% of FEED2 was added on days 4, 6, 8, 10 and 12. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.
CHO-GS (CHOZN®) cell line
Fed-batch culture of CELLiST BASAL3 / BASAL10 with FEED2
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against six popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 7, 9, and 11. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.
CHO-DG44 cell line
Fed-batch culture of CELLiST BASAL3 with FEED2
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+FEED2 was evaluated against eight popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 7, 9, and 11. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.
CHO-S cell line
Fed-batch culture of CELLiST BASAL3 / BASAL10 with FEED2
A Viable cell density
B IgG Titer (Day 14)
CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against five popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 6, 8, and 12. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.
| Documents & Links for CELLiST Feed media | |
| Datasheet | CELLiST Feed media Datasheet - Google translated |
| Flyer | CELLiST Feed media Flyer |
| Manual | CELLiST Feed media Manual |
| Documents & Links for CELLiST Feed media | |
| Datasheet | CELLiST Feed media Datasheet - Google translated |
| Flyer | CELLiST Feed media Flyer |
| Manual | CELLiST Feed media Manual |