CELLiST Basal media BASAL3

Cosmo Bio LTD

Catalog No: AJI-BASAL3-1

CELLiST™ BASAL Media

CELLiST™ BASAL Media are all Chemically Defined Media, optimized for Chinese Hamster Ovary (CHO) cells cultures and the production of recombinant proteins. CELLiST™ BASAL Media are developed based on Ajinomoto's capabilities in cell culture media development and know-how of amino acids science. CELLiST™ BASAL Media can be applied to various CHO cell lines, including CHO-K1, CHO-S, CHO-DG44 and CHO-DXB11.

Features

• Chemically formulated and free from animal-derived ingredients
• Contains high-quality amino acids based on the Ajinomoto's long-standing amino acid research capabilities and expertise
• Suitable for most CHO cell lines including CHO-K1, CHO-GS, CHO-S, CHO-DG44 and CHO-DXB11
• Packaging form can be adjusted according to customer’s requirements

Specifications

Model No.	BASAL3
TYPE	Powder
Concentration	27.0g / L
With Additives	D-Glucose
Storage Condition	2C to 8C, dark and dry
Shelf Life	36 months
Item Description	Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

 

Model No.	BASAL3P
TYPE	Powder
Concentration	28.0g / L
With Additives	D-Glucose · Poloxamer
Storage Condition	2C to 8C, dark and dry
Shelf Life	24 months
Item Description	Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Performance

CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3 with CELLiST F7

A Viable cell density

B IgG Titer (Day 14)

Figure 1: Fed-batch process was performed in an Ambr®15 microbioreactor system, using CHO-K1 cell line expressing IgG1 antibody. Feeding in CELLiSTTM group was performed at a concentration of 6% (v/v) on day 3, 5, 7, 9 and 11. ‘Supplier A, B, C, D and E’ represent basal/feed media combinations from major media suppliers. Culture and feeding manners were performed according to each manufacturer’s recommendations.

Protein Quality Across Different Scales

Fed-batch process was performed in 125 mL Erlenmeyer flasks, Ambr®15 microbioreactor system, and 2L single-use bioreactor system (Sartorius, BioStat® B), using CHO-DG44, CHO-S and CHO-GS cell lines expressing IgG1 antibody. Feeding was performed at a concentration of 6% (v/v) on day 4, 6, 8, 10 and 12.