CELLiST Basal media BASAL10

Cosmo Bio LTD

Catalog No: AJI-BASAL10-1

CELLiST™ BASAL Media

CELLiST™ BASAL Media are all Chemically Defined Media, optimized for Chinese Hamster Ovary (CHO) cells cultures and the production of recombinant proteins. CELLiST™ BASAL Media are developed based on Ajinomoto's capabilities in cell culture media development and know-how of amino acids science. CELLiST™ BASAL Media can be applied to various CHO cell lines, including CHO-K1, CHO-S, CHO-DG44 and CHO-DXB11.

Features

• Chemically formulated and free from animal-derived ingredients
• Contains high-quality amino acids based on the Ajinomoto's long-standing amino acid research capabilities and expertise
• Suitable for most CHO cell lines including CHO-K1, CHO-GS, CHO-S, CHO-DG44 and CHO-DXB11
• Packaging form can be adjusted according to customer’s requirements

Specifications

Model No.	BASAL10
TYPE	Powder
Concentration	24.3g / L
With Additives	D-Glucose
Storage Condition	2C to 8C, dark and dry
Shelf Life	36 months
Item Description	Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Model No.	BASAL10P
TYPE	Powder
Concentration	25.3g / L
With Additives	D-Glucose · Poloxamer
Storage Condition	2C to 8C, dark and dry
Shelf Life	24 months
Item Description	Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Performance

CHO-K1 cell line
Fed-batch culture of CELLiST BASAL10 with CELLiST F7

A Viable cell density

B IgG Titer (Day 14)

Figure 1. Fed-batch process was performed in a 125 mL Erlenmeyer flask system, using CHO-K1 cell line expressing IgG1 antibody. Feeding in CELLiSTTM group was performed at a concentration of 6% (v/v) on day 4, 6, 8, 10 and 12. A, A2, B and C represent basal/feed media combinations from major media suppliers. Culture and feeding manners were performed according to each manufacturer’s recommendations.