BroadCheck Metallothionein kit

Cosmo Bio

Catalog No.: NMD-MSFR077610

This ELISA kit is an indirect competitive reaction assay which uses an antibody that specifically recognizes metallothionein.

Background

Metallothionein (MT) is a metal-binding protein discovered by Margoshes and Vallee in 1957. It is widely known to be involved in metal metabolism and detoxification of heavy metals in the body. In recent years, it has also been attracting attention in research on cancer, immunity, stress, and Alzheimer's.

*Reference: J.Am.Chem.Soc . (1957) 79, P4813-4814

Features

Breakthrough!!
You can measure the amount of metallothionein (MT) protein in the body.
Quantitative measurement:
MT-I and MT-II can be quantified simultaneously.　 World 's first
High cross-reactivity
This kit alone can measure all animal species used in MT experiments (mouse, rat, rabbit, human, fish, etc.)

A wide range of samples can be measured, including blood, tissue, urine, and cultured cells, and the amount of MT-I and II in each sample can be accurately measured.

Kit Components

IgG immobilized plate (strip type)
Metallothionein standard (prepared)
Standard Diluent
antibody solution
Second antibody solution
Secondary antibody dilution
enzyme substrate solution
Stop solution
Concentrated cleaning solution

Measurement principle

This ELISA kit is an indirect competitive reaction assay using an antibody that specifically recognizes metallothionein. Metallothionein is immobilized in each well of the plate. A competitive reaction occurs by sequentially adding the reagents in the kit and the sample to each well. When a second antibody is then added, an HRP-bound antibody-metallothionein complex is formed. The amount of this complex depends on the amount of metallothionein contained in the sample to be measured. Finally, the metallothionein concentration in the sample can be determined by measuring the enzyme (HRP) activity of this complex.