Bone Resorption Assay Kit

Catalog No:
CSR-BRA-24KIT
$676.00

Bone Resorption Assay Kit is for measurement of cellular bone resorption activity.

Bone resorption activity releases fluoresceinamine-labeled chondroitin sulfate from plate-bound calcium phosphate and is quantitated by spectrophotometric evaluation of culture media fluorescence intensity.

Background
Useful for the research of skeletal metabolism This product is an assay kit for the measurement of bone resorption activity using a fluoresceinated calcium phosphate-coated plate (patent pending). The coated calcium phosphate is first bound to fluoresceinamine-labeled chondroitin sulfate (FACS), which is released from the calcium phosphate layer into conditioned medium by osteoclastic resorption activity. Bone resorption activity is evaluated by simply measuring the fluorescence intensity of the conditioned medium. This assayprovides a rapid evaluation system unlike that of the traditional pit assay

Features
1) Bone resorption activity is evaluated simply by measuring the fluorescence intensity of the medium.
2) The fluorescence excitation and emission wavelengthsare identical to those for the commonly-used fluorescent dye, FITC
3) Microscopic observation of cell morphology is possible.
4) Pit area analysis is also possible after removing the cells.
Ready to use, sterile components.

References (CSR-BRA-24P、CSR-BRA-48P)
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Documents & Links for Bone Resorption Assay Kit
Datasheet Bone Resorption Assay Kit Datasheet
Flyer Bone Resorption Assay Kit Flyer
Vendor Page Bone Resorption Assay Kit at Cosmo Bio LTD

Documents & Links for Bone Resorption Assay Kit
Datasheet Bone Resorption Assay Kit Datasheet
Flyer Bone Resorption Assay Kit Flyer
Vendor Page Bone Resorption Assay Kit

Citations for Bone Resorption Assay Kit – 11 Found
Cheng, Tsung-Lin; Lai, Chao-Han; Shieh, Shyh-Jou; Jou, Yin-Bo; Yeh, Jwu-Lai; Yang, Ai-Lun; Wang, Yan-Hsiung; Wang, Chau-Zen; Chen, Chung-Hwan; Shi, Guey-Yueh; Ho, Mei-Ling; Wu, Hua-Lin. Myeloid thrombomodulin lectin-like domain inhibits osteoclastogenesis and inflammatory bone loss. Scientific Reports. 2016;6( 27311356):28340.  PubMed
Park, Sil; Kanayama, Keiichi; Kaur, Kawaljit; Tseng, Han-Ching Helen; Banankhah, Sina; Quje, Davood Talebi; Sayre, James W; Jewett, Anahid; Nishimura, Ichiro. Osteonecrosis of the Jaw Developed in Mice: DISEASE VARIANTS REGULATED BY γδ T CELLS IN ORAL MUCOSAL BARRIER IMMUNITY. The Journal Of Biological Chemistry. 2015;290(28):17349-66.  PubMed
Kang, J H; Ko, H M; Moon, J S; Yoo, H I; Jung, J Y; Kim, M S; Koh, J T; Kim, W J; Kim, S H. Osteoprotegerin expressed by osteoclasts: an autoregulator of osteoclastogenesis. Journal Of Dental Research. 2014;93(11):1116-23.  PubMed
Kim, Yun-Ho; Kim, Jung-Lye; Lee, Eun-Jung; Park, Sin-Hye; Han, Seon-Young; Kang, Soon Ah; Kang, Young-Hee. Fisetin antagonizes cell fusion, cytoskeletal organization and bone resorption in RANKL-differentiated murine macrophages. The Journal Of Nutritional Biochemistry. 2014;25(3):295-303.  PubMed
Lee, Chang Won; Seo, Jeong Yeon; Choi, Ji Won; Lee, Jisun; Park, Joo Woong; Lee, Jae Yeon; Hwang, Kyo-Yeol; Park, Young Shik; Park, Yong Il. Potential anti-osteoporotic activity of low-molecular weight hyaluronan by attenuation of osteoclast cell differentiation and function in vitro. Biochemical And Biophysical Research Communications. 2014;449(4):438-43.  PubMed
Kuo, Chih-Hsin; Chen, Kuei-Fang; Chou, Shah-Hwa; Huang, Ya-Fang; Wu, Cheng-Ying; Cheng, Da-En; Chen, Yu-Wen; Yang, Chih-Jen; Hung, Jen-Yu; Huang, Ming-Shyan. Lung tumor-associated dendritic cell-derived resistin promoted cancer progression by increasing Wolf-Hirschhorn syndrome candidate 1/Twist pathway. Carcinogenesis. 2013;34(11):2600-9.  PubMed
Hsu, Li-Chien; Reddy, Sakamuri V; Yilmaz, Özlem; Yu, Hong. Sphingosine-1-Phosphate Receptor 2 Controls Podosome Components Induced by RANKL Affecting Osteoclastogenesis and Bone Resorption. Cells. 2019;8(1)  PubMed
Ma, Yong; Wang, Lining; Zheng, Suyang; Xu, Jiake; Pan, Yalan; Tu, Pengcheng; Sun, Jie; Guo, Yang. Osthole inhibits osteoclasts formation and bone resorption by regulating NF-κB signaling and NFATc1 activations stimulated by RANKL. Journal Of Cellular Biochemistry. 2019;120(9):16052-16061.  PubMed
Lu, Jiajia; Shi, Xiaojian; Fu, Qiang; Han, Yaguang; Zhu, Lei; Zhou, Zhibin; Li, Yongchuan; Lu, Nan. New mechanistic understanding of osteoclast differentiation and bone resorption mediated by P2X7 receptors and PI3K-Akt-GSK3β signaling. Cellular & Molecular Biology Letters. 2024;29(1):100.  PubMed
Gal, Minju; Tuan, Ha Manh; Park, Ju-Hee; Park, Kang-Hyeon; Kim, Okhwa; Min, Byung-Sun; Lee, Jeong-Hyung. Irilin D suppresses RANKL-induced osteoclastogenesis and prevents inflammation-induced bone loss by disrupting the NF-κB and MAPK signaling pathways. European Journal Of Pharmacology. 2024;982:176956.  PubMed
Xue, Peng; Zhang, Weixin; Shen, Mengxi; Yang, Junhua; Chu, Jiachen; Wang, Shenyu; Wan, Mei; Zheng, Junying; Qiu, Zhaozhu; Cao, Xu. Proton-Activated Chloride Channel Increases Endplate Porosity and Pain in a Mouse Spinal Degeneration Model. The Journal Of Clinical Investigation. 2024; 39196784  PubMed