Atelocollagen Honeycomb sponge

Cosmo Bio

SKU:
KOU-CSH-10
  • Atelocollagen Honeycomb sponge
  • Atelocollagen Honeycomb sponge
  • Atelocollagen Honeycomb sponge
  • Atelocollagen Honeycomb sponge
  • Suppression of teratoma formation by embryoid body (EB) following transplantation of EB on Honeycomb Sponge (CSH) (Yamaki M, Harvard University, Stem Cell Regenerative Biology) Mouse kidney at 12 weeks following transplantation of EB on CSH scaffold (EB+CSH) barely showed sign of teratoma formation while EB transplanted without CSH (EB alone) generated teratomas in all mice. Histologically, transplanted EB+CSH were indistinguishable from adjacent host renal tissue, suggesting spontaneous differentiation without specific induction. Methods: Plate cultured mouse embryonic stem (ES) cells were trypsinized, filtered through nylon mesh, seeded into 96-well plates (1x104 cells/well), and cultured for 5 days to form embryoid bodies. When EB were mixed with CSH, They were rapidly and uniformly integrated into the CSH matrix. EB+CSH complex was then transplanted under renal capsule of 6-week-old mice (Ref. 7).
  • 3D culture of vascular smooth muscle cells (SMCs) using CSH (Ishii I, Graduate School of Pharmaceutical Sciences, Chiba University) It has been reported that mouse SMCs cultured in CSH turn to differentiated state from dedifferentiated state and also the proliferation is suppressed (Ref. 6). In order to investigate the mechanism of proliferative inhibition, p27 deficient mouse derived SMCs and p21Cip1 siRNA were used but the effect on proliferation was observed only in SMCs cultured on plates. Although further research is required to clarify the mechanism, SMCs cultured in CSH can maintain similar features to in vivo aortic SMCs for more than one month and therefore it may be useful for regenerative medicine (Ref. 1).
  • Administration of medical plant extract and cell migration using CSH (Maeda A, Ph. D. Skin Regeneration, PIAS Collaborative Research, Osaka University) he ethanol extract from Mallotus philippinensis bark (EMPB) is impregnated to CSH and topically applied to the wound of mouse. Then, luciferase-expressing MSCs (Luc-MSCs) were intravenously injected. As a result, accumulation of Luc-MSCs at the wound area was promoted in EMPB treated group. Further evaluations at 17 days after EMPB administration to the wound area revealed that accelerated wound healing and increased numbers of capillaries and granulation tissue. These results suggests that CSH is useful for sustained release of bioactive substance (Ref. 5).
  • Implantation of bone marrow stem cells (BMSCs) into hemisected spinal cord using CSH (Enomoto M, Department of Orthopaedic Surgery and Hyperbaric Medical Center, Tokyo Medical and Dental University) A previous report demonstrated that the pore structure of CSH enhances nerve regeneration (Ref. 9). In this research, a remarkable increase of neurite growth was observed when dorsal root ganglia (DRG)s were cultured on GEP-expressing BMSCs contained CSH (BMSCs+CSH) for ten days compared to DRGs cultured on CSH alone. Improved locomotor and sensory function was also observed four weeks after implantation of BMSC+CSH into hemisected spinal cord (Ref. 4). [Tuj-1: A maker for neuron; SMI31: A marker for neurofilament; GFAP: a marker for astrocyte; BBB score: an evaluation method for hindlimb motor function]
  • Induction of hepatic differentiation of ES cells and transplantation using CSH Embryoid bodies (EBs) were formed from ES cells and then inserted into CSH. EBs both with and without CSH were cultured to differentiate and induce hepatic histogenesis. The EB-derived cells expressed liver-specific genes, and albumin-positive cells formed cordlike structures that were not present in those without CSH. The scaffold including EB-derived hepatocyte-like cells was transplanted into the median lobe of mice. After 14 days, cells positive for both albumin and cytokeratin 18 appeared in the transplant and formed clustered aggregates. (Ref. 10) [AFP: Marker for inicial livers hepatic cells; ALB: Marker for inicial to matured hepatic cells; G6P and CK18: Marker for matured hepatic cells]
  • Atelocollagen Honeycomb sponge
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Background
Honeycomb Sponge is made from type I atelocollagen and possess honeycomb-like pore structure with high pore density. This honeycomb structure enables easy supply of nutrients to cells and excretion of waste products from cells.

Applications

  • 3D cell culture
  • Scaffold for regenerative medicine research
  • Sustained release of bioactive substances

Features

  • Unidirectional pore structure facilitates cell migration and vascularization in vivo
  • Cells can be easily harvested by collagenase treatment.

This product is manufactured in Japan and produced from raw materials obtained from livestock certified born and raised in Australia or New Zealand. Japan, Australia and New Zealand are all recognized by the World Health Organization as having negligible risk for Bovine Spongiform Encephalitis (BSE). Nevertheless, please consult with your country's Customs office to confirm importability.





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