Three-dimensional culture of blood vessel endothelium using the collagen gel (Eguchi R, Hyogo College of Medicine Department of Environmental and Preventive Medicine) An immunosuppressant FK506 is known to be involved in endothelial dysfunction inducing thrombotic microangiopathy after hematopoietic stem cell transplantation. In order to clarify the mechanism of the event, human umbilical vein endothelial cells (HUVEC) in collagen gel 3D culture were treated with FK506. In 3D culture, FK506 induced cell death and tube structure breakdown in a time- and concentration-dependent manner, but showed little effect on cells in monolayer culture. These results suggest 3D culture in collagen gel is useful to investigate in vivo phenomenon in vitro (Ref.6).

Atelocollagen, DMEM High Glucose

Catalog No:
KOU-DME-02H
$131.00

ATTENTION US CUSTOMERS: We will fully subsidize the import permit of qualified first-time users. FREE samples may be available for 50% shipping fee. Contact us for information about permits and samples.

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Background
These products are prepared with highly purified type I collagen derived from bovine dermis. Neutral Solution products are pH neutral, come premixed in several commonly used culture media, and gelate at 37°C. In contrast to cell-derived soluble basement membrane preparations, Collagen Solutions are free from bioactive substances, nucreic acids, MMP, etc. allowing experimental results to be evaluated more clearly.

Applications

  • Collagen coating for cell cultureware
  • Cell culture in a collagen gel
  • Cell culture on a collagen gel

Features

  • Solutions gelate at 37°C
  • Ready-to-use: atelocollagen is premixed with medium

This product is manufactured in Japan and produced from raw materials obtained from livestock certified born and raised in Australia or New Zealand. Japan, Australia and New Zealand are all recognized by the World Health Organization as having negligible risk for Bovine Spongiform Encephalitis (BSE). Nevertheless, please consult with your country's Customs office to confirm importability.

Documents & Links for Atelocollagen, DMEM High Glucose
Datasheet Atelocollagen, DMEM High Glucose Datasheet
Flyer Atelocollagen, DMEM High Glucose Flyer
Vendor Page Atelocollagen, DMEM High Glucose at Cosmo Bio LTD

Documents & Links for Atelocollagen, DMEM High Glucose
Datasheet Atelocollagen, DMEM High Glucose Datasheet
Flyer Atelocollagen, DMEM High Glucose Flyer
Vendor Page Atelocollagen, DMEM High Glucose