Anti SUZ12 mAb (Clone 1B10)

CUSABIO

Catalog No.:
CSB-RA159561A0HU-50
Western Blot<br /> Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate, THP-1 whole cell lysate, K562 whole cell lysate<br /> All lanes: SUZ12 antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 84 kDa<br /> Observed band size: 90 kDa<br />
IHC image of CSB-RA159561A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating SUZ12 in K562 whole cell lysate<br /> Lane 1: Rabbit control IgG instead of CSB-RA159561A0HU in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br /> Lane 2: CSB-RA159561A0HU(2µg)+ K562 whole cell lysate(500µg)<br /> Lane 3: K562 whole cell lysate (10µg)<br />
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Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

Product Specifications
Application WB, IHC, IP, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 1B10)

Documents & Links for Anti SUZ12 mAb (Clone 1B10)
Datasheet Anti SUZ12 mAb (Clone 1B10) Datasheet

Documents & Links for Anti SUZ12 mAb (Clone 1B10)
Datasheet Anti SUZ12 mAb (Clone 1B10) Datasheet
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