Anti SF3B1 mAb (Clone 6C7)

CUSABIO

Catalog No.:
CSB-RA102629A0HU-50
Western Blot<br /> Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, U-87 whole cell lysate, Mouse Brain whole cell lysate<br /> All lanes: SF3B1 antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 146, 17 kDa<br /> Observed band size: 130 kDa<br />
IHC image of CSB-RA102629A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA102629A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela Cells with CSB-RA102629A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Product Specifications
Application WB, IHC, IF, ELISA
Reactivity Human, Mouse
Clonality Monoclonal (Clone No.: 6C7)

Documents & Links for Anti SF3B1 mAb (Clone 6C7)
Datasheet Anti SF3B1 mAb (Clone 6C7) Datasheet

Documents & Links for Anti SF3B1 mAb (Clone 6C7)
Datasheet Anti SF3B1 mAb (Clone 6C7) Datasheet
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