Anti-SARS-CoV-2 Spike glycoprotein Recombinant Chimeric Antibody

Cosmo Bio LTD

Catalog No: HAK-ANTI-SPD-CAB-1

When the new coronavirus infects a cell, the spike protein on the surface of the virus binds to ACE2 on the cell surface. Drugs that block this binding will prevent viral infection.

1. ACE2 extracellular domain
2. SARS-CoV-2 spike protein RBD/rabbit Fc tag
3. Biotinylated SARS-CoV-2 spike protein RBD and rabbit Fc tag
4. Neutralizing antibodies

We have also started selling a monoclonal antibody ( anti-SARS-CoV-2 spike protein monoclonal antibody (mouse)

) that is specific and has high affinity to the receptor binding domain of the spike protein S1 domain . This is a neutralizing antibody that inhibits the binding of the receptor binding domain to ACE2. 

• We also offer mutant strains. Please see here .

 

SARS-CoV-2 spike protein RBD/rabbit Fc tag

Biotinylated SARS-CoV-2 spike protein RBD and rabbit Fc tag

background

Angiotensin-converting enzyme 2 (ACE2), also known as ACE homolog, is an integral membrane protein that is highly homologous to ACE. ACE2 was known as one of the factors that work in the renin-angiotensin system, which is involved in blood pressure regulation. Recently, it has been revealed that when the novel coronavirus that causes COVID-19 infects human cells, it binds to ACE2 present in the cell membrane and is then taken into the cell, and ACE2 is also thought to be a receptor for the novel coronavirus. ⁽¹⁾ A highly sensitive binding assay is provided by immobilizing
human ACE2 protein with His tag (product number: HAK-ACE2_UL-1) on a 96-well plate and adding SARS-CoV-2 spike protein RBD with rabbit Fc tag (product number: HAK-SPD_UL-1) or biotinylated SARS-CoV-2 spike protein RBD with rabbit Fc tag (product number: HAK-SPD_bio-1), which is a fusion protein in which the receptor binding domain of the viral protein is bound to rabbit Fc, and detecting with an HRP-labeled secondary antibody or streptavidin.

Protocol for binding experiment between SARS-CoV-2 spike protein and ACE2

Detailed protocols for binding assays and competitive inhibitor screening are available. You can download the PDF
here .

Anti-SARS-CoV-2 Spike Protein Recombinant Chimeric Antibody (Human/Mouse)

Product Information

Antibody Information
Clone name: A2-5A12H Antibody details: Anti-SARS-CoV-2 Spike Protein Recombinant Chimeric Antibody (Human/Mouse) Class: IgG1/κ Immunogen: Recombinant SARS-CoV-2 spike protein (Val327~Thr531) Species of origin: human/mouse Applications: ELISA, spike protein/ACE2 binding inhibition experiments
concentration
Approximately 1mg/mL
composition
0.1M-PBS (pH 7.2~7.4), 0.2µm filter
keep
Avoid repeated freeze-thaw cycles. Upon receipt, store product at -70°C or colder. Aliquoting is recommended prior to use.

 

Usage Example

1. Inhibition of binding between spike protein and ACE2
• Biotin-labeled RBD protein diluted to 500ng/mL and the binding inhibitor anti-SARS-CoV-2 spike protein recombinant chimeric antibody (human/mouse) (0.8-1000ng/mL) were added to a 96-well microplate with ACE2 protein immobilized, at 50µL per well, and incubated at room temperature for 2 hours. Mouse anti-SARS-CoV-2 spike protein antibody (product number: HAK-ANTI-SPD-MAB-1), an original clone sold separately, was used as a comparison antibody.
  After reaction with each antibody, the plate was washed, and HRP-labeled streptavidin and color-developing substrate were added to react. After adding a stop solution, the absorbance of each well was measured.
  As a result, both antibodies strongly inhibited the binding of the RBD protein to ACE2, and the IC50 values ​​of this product's recombinant chimeric antibody and the separately sold mouse anti-SARS-CoV-2 spike protein antibody (product number: HAK-ANTI-SPD-MAB-1) were 10.3ng/mL and 14.4ng/mL, respectively. The reaction diagram and binding inhibitory activity are shown below.

Fig. 9 Reaction diagram

Figure 10. Binding inhibition activity