Anti NKX2-1 mAb (Clone 6C11)

CUSABIO

SKU:
CSB-RA181189A0HU-50
  • Western Blot<br /> Positive WB detected in: HepG2 whole cell lysate, U251 whole cell lysate, U87 whole cell lysate<br /> All lanes: TTF1 antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 39 kDa<br /> Observed band size: 39 kDa<br />
  • IHC image of CSB-RA181189A0HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-RA181189A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Overlay histogram showing A549 cells stained with CSB-RA181189A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).

Product Specifications
Application WB, IHC, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 6C11)


Documents & Links for Anti NKX2-1 mAb (Clone 6C11)
Datasheet Anti NKX2-1 mAb (Clone 6C11) Datasheet
Vendor Page Anti NKX2-1 mAb (Clone 6C11) at CUSABIO

Documents & Links for Anti NKX2-1 mAb (Clone 6C11)
Datasheet Anti NKX2-1 mAb (Clone 6C11) Datasheet
Vendor Page Anti NKX2-1 mAb (Clone 6C11)



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