Neurocan is a nervous tissue-unique, secretory proteoglycan that carries predominantly chondroitin sulfate side chains. Its expression gradually decreases with nervous tissue development. In the immature brain, neurocan exists in a full-length form with a 240 kDa-core glycoprotein, whereas in the mature brain it exists as proteolytic fragments of the NH2-terminal half (neurocan-N) with a 130 kDa-core glycopeptide and the COOH-terminal half (neurocan-C) with a 150 kDa-core glycopeptide. Neurocan is implicated in neural network formation and is a susceptibility factor for bipolar disorder. It is upregulated in central nervous system lesion sites and is a major component of glial scars. This antibody effectively recognizes the COOH-terminal half of rat neurocan core glycoprotein as well as the full length neurocan core glycoprotein.
References:
1) Oohira, A. et al. (1994) Neuroscience. 60:145-157. PMID: 8052408.
2) Matsui, F. et al. (1994) Neurochem. Int. 25:425-431.
3) Watanabe, E. et al. (1995) Eur. J. Neurosci. 7:547-554.
4) Fukuda, T. et al. (1997) J. Comp. Neurol. 382:141-152.
5) Inatani, M. et al. (1999) Invest. Ophthalmol. Vis. Sci. 40:2350-2359.
Product Specifications
Application
IP, IHC(p), WB, ELISA, IHC(f)
Reactivity
Rat
Clonality
Monoclonal (Clone No.: 1G2)
Host
Mouse
Documents & Links for Anti Neurocan (NCAN) mAb (Clone 1G2)
![Characterization of neurocan peptide pAb and neurocan mAb (clone 1G2) with samples obtained from 10-day-old rat brain. PBS-soluble brain CSPG mixture of 10-day-old rats (lane 1) and brain PBS-extract digested with chondroitinase ABC (lanes 2 and 3) were separated by SDS-PAGE. Neurocan peptide pAb recognized a very diffuse band with the same mobility as that recognized by neurocan mAb (clone 1G2). Monoclonal antibody (clone 1G2) recognized both 240 and 150 kDa core glycoproteins in the chondroitinase-digested sample. The polyclonal antibody recognized not only the 240 kDa core glycoprotein but also one with a molecular weight of 130 kDa. [from: Neurochem. Int. (1994) 25:425-431.]](https://cdn11.bigcommerce.com/s-ydswqc5qsc/images/stencil/500x659/products/29732/120198/cac-nu-07-002_anti-neurocan-mab-clone-1g2_1306__20473.1620917124.png?c=2 "Characterization of neurocan peptide pAb and neurocan mAb (clone 1G2) with samples obtained from 10-day-old rat brain. PBS-soluble brain CSPG mixture of 10-day-old rats (lane 1) and brain PBS-extract digested with chondroitinase ABC (lanes 2 and 3) were separated by SDS-PAGE. Neurocan peptide pAb recognized a very diffuse band with the same mobility as that recognized by neurocan mAb (clone 1G2). Monoclonal antibody (clone 1G2) recognized both 240 and 150 kDa core glycoproteins in the chondroitinase-digested sample. The polyclonal antibody recognized not only the 240 kDa core glycoprotein but also one with a molecular weight of 130 kDa. [from: Neurochem. Int. (1994) 25:425-431.]")
