Anti MSR-A (CD204) mAb (Clone SRA-E5, IgG1)

Cosmo Bio LTD

Catalog No: KAL-KT022-T

Click here to view our "KAL-xxxxx" products dashboard page

Macrophage scavenger receptor (MSR-A: CD204) was identified in the search for the receptor molecules that are implicated in the pathological deposition of cholesterol during atherogenesis through receptor-mediated uptake of modified low density lipoproteins (LDL). MSR-As possess a wide range of ligand-binding specificities and recognize a variety of molecules such as modified LDL including acetylated LDL, oxidized LDL, advanced glycation end products (AGE), polyribonucleotides such as poly G and poly I and bacterial surface lipids including lipopolysaccharide and lipoteicoic acid. This antidody was produced from the mouse immunized with recombinant protein of human type I MSR-A ,and has been proved to be useful for the immunoblotting and immunohistochemistry. This antibody is useful tools for the study of MSR in atherogenesis and various other pathological conditions in humans and animal species.

Package Size 50 g   (200 l / vial) 
Format  Mouse monoclonal antibody 0.25mg/ml 
Buffer Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat 
Storage  Store below -20 
Clone No.  SRA-E5 Subclass IgG1

 Purification method The spleen cells from mouse, immunized with recombinant protein of human type I SR-A, were fused with mouse NS-1 myeloma cells. The hybridoma cell line with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.

Working dilution for immunohistochemistry: 10 g/ml , for immunoblotting : 2 g/ml Antigen retrieval (microwave 10 min , 0.01M citrate buffer, ph2.0) is recommended for paraffin sections.

Reference
1. Takeya M., Tomokiyo R., Jinnouchi K., Sakaguchi H., et al: Macrophage Scavenger Receptors: Structure, Function and Tissue Distribution: Acta Histochem. Cytochem. 32(1):47-51,1999
2. Tomokiyo R., Jinnouchi K., Honda M., Wada Y., Hanada N., Hiraoka T., Suzuki H., Kodama T., Takahashi K., Takeya M.: Production, characterization, and interspecies reactivities of monoclonal antibodies against human class A macrophage scavenger receptors: Atherosclerosis, 2001, in press.
3. Takeya M., Tomokiyo R., Jinnouchi K. Honda M. et al: CD204: Macrophage scavenger receptor, a new differentiation marker for macrophages: part 9 Myeloid Cells, New CD Antigens, Leucocyte Typing , Oxford Univ. Press, 2001, in press. 

Citations for Anti MSR-A (CD204) mAb (Clone SRA-E5, IgG1) – 12 Found

Maile, Laura A; Busby, Walker H; Nichols, Timothy C; Bellinger, Dwight A; Merricks, Elizabeth P; Rowland, Michael; Veluvolu, Umadevi; Clemmons, David R. A monoclonal antibody against alphaVbeta3 integrin inhibits development of atherosclerotic lesions in diabetic pigs. Science Translational Medicine. 2010;2(18):18ra11. 

Withers, Sita S; York, Daniel; Choi, Jin W; Woolard, Kevin D; Laufer-Amorim, Renee; Sparger, Ellen E; Burton, Jenna H; McSorley, Stephen J; Monjazeb, Arta M; Murphy, William J; Canter, Robert J; Rebhun, Robert B. Metastatic immune infiltrates correlate with those of the primary tumour in canine osteosarcoma. Veterinary And Comparative Oncology. 2019;17(3):242-252. 

Yamane, Taishi; Kanamori, Yohei; Sawayama, Hiroshi; Yano, Hiromu; Nita, Akihiro; Ohta, Yudai; Hinokuma, Hironori; Maeda, Ayato; Iwai, Akiko; Matsumoto, Takashi; Shimoda, Mayuko; Niimura, Mayumi; Usuki, Shingo; Yasuda-Yoshihara, Noriko; Niwa, Masato; Baba, Yoshifumi; Ishimoto, Takatsugu; Komohara, Yoshihiro; Sawa, Tomohiro; Hirayama, Tasuku; Baba, Hideo; Moroishi, Toshiro. Iron accelerates Fusobacterium nucleatum-induced CCL8 expression in macrophages and is associated with colorectal cancer progression. Jci Insight. 2022;7(21) 
- Comments: see Supplementary Information https://df6sxcketz7bb.cloudfront.net/manuscripts/156000/156802/jci.insight.156802.sd.pdf

Ikezumi, Yohei; Suzuki, Toshiaki; Karasawa, Tamaki; Hasegawa, Hiroya; Yamada, Takeshi; Imai, Naofumi; Narita, Ichiei; Kawachi, Hiroshi; Polkinghorne, Kevan R; Nikolic-Paterson, David J; Uchiyama, Makoto. Identification of alternatively activated macrophages in new-onset paediatric and adult immunoglobulin A nephropathy: potential role in mesangial matrix expansion. Histopathology. 2011;58(2):198-210. 

Irie, Tomoaki; Yoshii, Daiki; Komohara, Yoshihiro; Fujiwara, Yukio; Kadohisa, Masashi; Honda, Masaki; Suzu, Shinya; Matsuura, Toshiharu; Kohashi, Kenichi; Oda, Yoshinao; Hibi, Taizo. IL-34 in hepatoblastoma cells potentially promote tumor progression via autocrine and paracrine mechanisms. Cancer Medicine. 2022;11(6):1441-1453. 

Tada, Yoichiro; Matsumi, Yoshiaki; Hara, Kazushi; Miyauchi, Wataru; Sugesawa, Ken; Uejima, Chihiro; Tanio, Akimitsu; Kihara, Kyoichi; Yamamoto, Manabu; Takano, Shuichi; Sakamoto, Teruhisa; Nakayama, Yuji; Hasegawa, Toshimichi; Fujiwara, Yoshiyuki. Infiltration of CD204-overexpressing Macrophages Contributes to the Progression of Stage II and III Colorectal Cancer. Anticancer Research. 2021;41(10):4857-4865. 

Maile, L A; Busby, W H; Xi, G; Gollahan, K P; Flowers, W; Gafbacik, N; Gafbacik, S; Stewart, K; Merricks, E P; Nichols, T C; Bellinger, D A; Clemmons, D R. An anti-αVβ3 antibody inhibits coronary artery atherosclerosis in diabetic pigs. Atherosclerosis. 2017;258( 28189040):40-50. 

Renshaw, Randall W; Griffing, Jennifer; Weisman, Jaime; Crofton, Lisa M; Laverack, Melissa A; Poston, Robert P; Duhamel, Gerald E; Dubovi, Edward J. Characterization of a Vesivirus Associated with an Outbreak of Acute Hemorrhagic Gastroenteritis in Domestic Dogs. Journal Of Clinical Microbiology. 2018;56(5) 

Maile, Laura A; Busby, Walker H; Nichols, Timothy C; Bellinger, Dwight A; Merricks, Elizabeth P; Rowland, Michael; Veluvolu, Umadevi; Clemmons, David R. A monoclonal antibody against alphaVbeta3 integrin inhibits development of atherosclerotic lesions in diabetic pigs. Science Translational Medicine. 2010;2(18):18ra11. 

Thompson, Mark A; Henderson, Kyle K; Woodman, Christopher R; Turk, James R; Rush, James W E; Price, Elmer; Laughlin, M Harold. Exercise preserves endothelium-dependent relaxation in coronary arteries of hypercholesterolemic male pigs. Journal Of Applied Physiology (Bethesda, Md. : 1985). 2004;96(3):1114-26. 

Yoshii, Daiki; Nakagawa, Takenobu; Komohara, Yoshihiro; Kawaguchi, Hiroaki; Yamada, Sohsuke; Tanimoto, Akihide. Phenotypic Changes in Macrophage Activation in a Model of Nonalcoholic Fatty Liver Disease using Microminipigs. Journal Of Atherosclerosis And Thrombosis. 2021;28(8):844-851. 

Motoshima, Takanobu; Miura, Yuji; Wakigami, Nanako; Kusada, Natsuki; Takano, Toshimi; Inoshita, Naoko; Okaneya, Toshikazu; Sugiyama, Yutaka; Kamba, Tomomi; Takeya, Motohiro; Komohara, Yoshihiro. Phenotypical change of tumor-associated macrophages in metastatic lesions of clear cell renal cell carcinoma. Medical Molecular Morphology. 2018;51(1):57-63.