Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703)

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SKU:
CAC-CTB-LC3-2-IC
  • Figure 1. Immunofluorescence microscopy analysis of mouse embryonic fibroblasts (MEFs). MEFs were cultured in DMEM supplemented with 10% FBS (left) or DMEM without amino acids (right) for 1 hr. After fixation with 4% paraformaldehyde for 10 min at room temperature, they were permeabilized with 50μg/ml digitonin for 5 min. Cells were then subjected to immunofluorescence microscopy using #1703 anti-LC3 antibody at 1:100 dilution. Scale bar, 20μm.
  • Figure 2. Immuno-electron microscopy analysis of mouse embryonic fibroblasts (MEFs). MEFs were cultured in DMEM without amino acids for 2 hr. After fixation with 4% paraformaldehyde for 2 hr at room temperature, they were permeabilized with liquid nitrogen. Immuno-electron microscopy analysis (pre-embedding method) of endogenous LC3 was performed using #1703 anti-LC3 antibody at 1:10 dilution. The gold labeling was intensified by using a silver enhancement kit (HQ silver enhancement kit, Nanoprobes, NY).
  • Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703)
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LC3 is a mammalian autophagosome marker. It is immediately cleaved to form LC3-I by Atg4, and when autophagy is induced, phosphatidylethanolamine is covalently linked to LC3-I C-terminal glycine to form LC3-II. LC3-II is membrane-bound and thought to be present in autophagosomal membranes. Since autophagosomes are degraded by fusion with lysosomes, LC3-II itself is also degraded by autophagy. Therefore, it is generally accepted that the amount of LC3-II correlates well with the amount of autophagosomes. This antibody shows good results in ICC and Immunoelectron microscopy experiments.

Source: Professor Noboru Mizushima, Department of Cell Physiology, Tokyo Medical and Dental University

References:
1) Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y. and Yoshimori, T. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing EMBO J. 19, 5720-5728. (2000)
2) Mizushima, N., Yoshimori, T. How to interpret LC3 immunoblotting Autophagy 3:542-545 (2007)
3) Mizushima, N., Yoshimori, T. and Levine, B. Methods in mammalian autophagy research. Cell 140; 313-326 (2010)



Product Specifications
Application ICC, IEM, IF
Reactivity Mouse, Human
Clonality Monoclonal (Clone No.: LC3-1703)
Host Mouse


Documents & Links for Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703)
Datasheet Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703) Datasheet

Documents & Links for Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703)
Datasheet Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703) Datasheet



Citations for Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703) – 9 Found
Murase, Daiki; Hachiya, Akira; Kikuchi-Onoe, Mamiko; Fullenkamp, Rachel; Ohuchi, Atsushi; Kitahara, Takashi; Moriwaki, Shigeru; Hase, Tadashi; Takema, Yoshinori. Cooperation of endothelin-1 signaling with melanosomes plays a role in developing and/or maintaining human skin hyperpigmentation. Biology Open. 2015;4(10):1213-21.  PubMed
Liang, Qiming; Seo, Gil Ju; Choi, Youn Jung; Kwak, Mi-Jeong; Ge, Jianning; Rodgers, Mary A; Shi, Mude; Leslie, Benjamin J; Hopfner, Karl-Peter; Ha, Taekjip; Oh, Byung-Ha; Jung, Jae U. Crosstalk between the cGAS DNA sensor and Beclin-1 autophagy protein shapes innate antimicrobial immune responses. Cell Host & Microbe. 2014;15(2):228-38.  PubMed
Yahiro, K; Akazawa, Y; Nakano, M; Suzuki, H; Hisatune, J; Isomoto, H; Sap, J; Noda, M; Moss, J; Hirayama, T. Helicobacter pylori VacA induces apoptosis by accumulation of connexin 43 in autophagic vesicles via a Rac1/ERK-dependent pathway. Cell Death Discovery. 1( 27551466):15035.  PubMed
Wijdeven, Ruud H; Janssen, Hans; Nahidiazar, Leila; Janssen, Lennert; Jalink, Kees; Berlin, Ilana; Neefjes, Jacques. Cholesterol and ORP1L-mediated ER contact sites control autophagosome transport and fusion with the endocytic pathway. Nature Communications. 2016;7( 27283760):11808.  PubMed
Lee, Seung Hee; Du, Jing; Stitham, Jeremiah; Atteya, Gourg; Lee, Suho; Xiang, Yaozu; Wang, Dandan; Jin, Yu; Leslie, Kristen L; Spollett, Geralyn; Srivastava, Anup; Mannam, Praveen; Ostriker, Allison; Martin, Kathleen A; Tang, Wai Ho; Hwa, John. Inducing mitophagy in diabetic platelets protects against severe oxidative stress. Embo Molecular Medicine. 2016;8(7):779-95.  PubMed
Kishi-Itakura, Chieko; Koyama-Honda, Ikuko; Itakura, Eisuke; Mizushima, Noboru. Ultrastructural analysis of autophagosome organization using mammalian autophagy-deficient cells. Journal Of Cell Science. 2014;127(Pt 18):4089-102.  PubMed
Yahiro, Kinnosuke; Tsutsuki, Hiroyasu; Ogura, Kohei; Nagasawa, Sayaka; Moss, Joel; Noda, Masatoshi. DAP1, a negative regulator of autophagy, controls SubAB-mediated apoptosis and autophagy. Infection And Immunity. 2014;82(11):4899-908.  PubMed
Jiang, Peidu; Nishimura, Taki; Sakamaki, Yuriko; Itakura, Eisuke; Hatta, Tomohisa; Natsume, Tohru; Mizushima, Noboru. The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Molecular Biology Of The Cell. 2014;25(8):1327-37.  PubMed
Liang, Qiming; Chang, Brian; Lee, Patrick; Brulois, Kevin F; Ge, Jianning; Shi, Mude; Rodgers, Mary A; Feng, Pinghui; Oh, Byung-Ha; Liang, Chengyu; Jung, Jae U. Identification of the Essential Role of Viral Bcl-2 for Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication. Journal Of Virology. 2015;89(10):5308-17.  PubMed
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