Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703)

Cosmo Bio LTD

Catalog No: CAC-CTB-LC3-2-IC

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Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.

Application: ICC, IEM, IF 
Clonality: Monoclonal 
Host: Mouse 
Purification: Ig-PG 
Reactivity: Mouse, Human 

LC3 is a mammalian autophagosome marker. It is immediately cleaved to form LC3-I by Atg4, and when autophagy is induced, phosphatidylethanolamine is covalently linked to LC3-I C-terminal glycine to form LC3-II. LC3-II is membrane-bound and thought to be present in autophagosomal membranes. Since autophagosomes are degraded by fusion with lysosomes, LC3-II itself is also degraded by autophagy. Therefore, it is generally accepted that the amount of LC3-II correlates well with the amount of autophagosomes. This antibody shows good results in ICC and Immunoelectron microscopy experiments.

Source: Professor Noboru Mizushima, Department of Cell Physiology, Tokyo Medical and Dental University

References:
1) Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y. and Yoshimori, T. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing EMBO J. 19, 5720-5728. (2000)
2) Mizushima, N., Yoshimori, T. How to interpret LC3 immunoblotting Autophagy 3:542-545 (2007)
3) Mizushima, N., Yoshimori, T. and Levine, B. Methods in mammalian autophagy research. Cell 140; 313-326 (2010)

Citations for Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703) – 9 Found

Cooperation of endothelin-1 signaling with melanosomes plays a role in developing and/or maintaining human skin hyperpigmentation.
Biol Open. 2015 Sep 4;4(10):1213-21. doi: 10.1242/bio.011973. 

Crosstalk between the cGAS DNA sensor and Beclin-1 autophagy protein shapes innate antimicrobial immune responses.
Cell Host Microbe. 2014 Feb 12;15(2):228-38. doi: 10.1016/j.chom.2014.01.009. 

Helicobacter pylori VacA induces apoptosis by accumulation of connexin 43 in autophagic vesicles via a Rac1/ERK-dependent pathway.
Cell Death Discov. 2015 Sep 28;1:15035. doi: 10.1038/cddiscovery.2015.35. eCollection 2015. 

Cholesterol and ORP1L-mediated ER contact sites control autophagosome transport and fusion with the endocytic pathway.
Nat Commun. 2016 Jun 10;7:11808. doi: 10.1038/ncomms11808. 

Inducing mitophagy in diabetic platelets protects against severe oxidative stress.
EMBO Mol Med. 2016 Jul 1;8(7):779-95. doi: 10.15252/emmm.201506046. Print 2016 Jul. 

Ultrastructural analysis of autophagosome organization using mammalian autophagy-deficient cells.
J Cell Sci. 2014 Sep 15;127(Pt 18):4089-102. doi: 10.1242/jcs.156034. Epub 2014 Jul 22. 

DAP1, a negative regulator of autophagy, controls SubAB-mediated apoptosis and autophagy.
Infect Immun. 2014 Nov;82(11):4899-908. doi: 10.1128/IAI.02213-14. Epub 2014 Sep 2. 

The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17.
Mol Biol Cell. 2014 Apr;25(8):1327-37. doi: 10.1091/mbc.E13-08-0447. Epub 2014 Feb 19. 

Identification of the Essential Role of Viral Bcl-2 for Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication.
J Virol. 2015 May;89(10):5308-17. doi: 10.1128/JVI.00102-15. Epub 2015 Mar 4.