Anti GA-Pyridine mAb (Clone 2A2)

Cosmo Bio LTD

Catalog No: CAC-AGE-M03

Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.

Background
Advanced Glycation End Product (AGE) is a general term for structures generated in the late stage of non-enzymatic glycation reactions (Millard reactions) between reducing sugars and proteins. AGE accumulation is known to be significantly elevated in age-related diseases such as diabetes and arteriosclerosis.

Glycolaldehyde formed as a result of the myeloperoxidase-H2O2 (MPO) reaction can react with proteins to yield various AGEs. Recently, a novel GA-derived AGE, called GA-pyridine, has been described in foam cells and the extracellular matrix of human atherosclerotic fibrotic lesions, glomerular mesangium and Bruch’s membrane and choroid. Since GA-pyridine significantly accumulates in foamy macrophages of atherosclerotic plaques, it is thought that the modification of proteins with glycolaldehyde (GA) is involved in the development of arteriosclerosis.

Source: Professor Nagai Ryuji, Tokai University Faculty of Agriculture Department of Biosciences Food Bioregulation Research Laboratory

Application

Western blotting, 1/200 to 1/400
Immunohistochemistry, 1/100 to 1/200
ELISA, 1/200 to 1/400

References

1) Nagai R., Hayashi CM., Xia L., Takeya M., Horiuchi S: Identification in human atherosclerotic lesions of GA-pyridine, a novel structure derived from glycolaldehyde-modified proteins. J Biol Chem. 277, 48905-48912 (2002). PMID: 12377783

2) Glenn JV., Mahaffy H., Wu K., Smith G., Nagai R., Simpson DAC., Boulton ME., Stitt AW. Advanced Glycation End Product (AGE) Accumulation on Bruch’s Membrane: Links to Age-Related RPE Dysfunction. Invest. Ophth. Vis. Sci. 50, 441-451 (2009). PMID: 18676633