Anti EIF4A1 mAb (Clone 7C3)

CUSABIO

Catalog No.:
CSB-RA190088A0HU-50
Western Blot<br /> Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, RAW264.7 whole cell lysate<br /> All lanes: eIF4A1 Antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 40, 47 kDa<br /> Observed band size: 50 kDa<br />
Immunofluorescence staining of Hela Cells with CSB-RA190088A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Product Specifications
Application WB, IF, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 7C3)

Documents & Links for Anti EIF4A1 mAb (Clone 7C3)
Datasheet Anti EIF4A1 mAb (Clone 7C3) Datasheet

Documents & Links for Anti EIF4A1 mAb (Clone 7C3)
Datasheet Anti EIF4A1 mAb (Clone 7C3) Datasheet
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