Check out our Human Collagen IVα Rat Monoclonal Antibodies Dashboard for more information about this product and additional epitope-defined monoclonal antibodies useful for research on hereditary diseases related to collagen IV, including our popular Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25) (SGE-CFT45325).
Alport syndrome is an inherited disease characterized by the pathological absence or reduction of the collagen α5(IV) chain in glomerular basement membrane (GBM), tubular basement membrane (TBM) and Bowman's capsular basement membrane. Anti Collagen 4 (Alpha 5) mAb (Clone H52) (cat no. SGE-C452) is an unlabeled rat monoclonal antibody specific for the α5 chain of collagen IV. It is useful in Western blot and indirect immunofluorescence experiments.
Product specifications
| Specification | Detail |
| Anti Collagen 4 (Alpha 5) mAb (Clone H52) |
| Cat No | SGE-C452 |
| Application | Staining of human cryostat sections by indirect immunofluorescence (acid-urea treatment is required) and Western blotting (dilution x10) |
| Quantity | 0.5 ml |
| Appearance | Solution |
| Purification | Hybridoma cell supernatant was diluted with culture medium containing 10% fetal bovine serum and 0.1% NaN3. |
| Fluorescent labeling | none |
| Clone name | H52 (rat IgG2b, kappa) |
| Specificity | H52 is specific to NC1 domain of α5(IV) (Reference 1). Reactive with NC1 domain of α5(IV) of rat, rabbit, guinea pig, dog, and cow. (Reference 1) |
| Antibodies preparation | Monoclonal antibody was prepared by the rat lymph node method developed by Shigei Medical Research Institute with a synthetic peptide of type IV collagen as immunogen. |
| Storage | Store below -30°C. Stable for several years under this condition. |
| Source | Shigei Medical Research Institute, 2117 Yamada, Okayama 701-0202, Japan; TEL: +81-86-282-3113; FAX: +81-86-282-3115; E-mail: inst@shigei.or.jp |
References
- Kagawa M et al.
Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport syndrome.
Nephrol. Dial. Transplant. 12: 1238-1241 (1997) (PMID: 9198058) - Borza DB et al.
The NCI domain of collagen IV encodes a novel network composed of the α1, α2, α5, and α6 chains in smooth muscle basement membranes.
J. Biol. Chem. 276: 28532-28540 (2001) (PMID: 11375996) - Sado et al.
Establishment by the rat lymph node method of epitope-defined monoclonal antibodies recognizing the six different α chains of human type IV collagen.
Histochem. Cell Biol. 104: 267-275 (1995) (PMID: 8548560) - Yoshioka K et al.
Type IV collagen α5 chain: Normal distribution and abnormalities in X-linked Alport syndrome revealed by monoclonal antibody.
Am. J. Pathol. 144: 986-996 (1994) (PMID: 8178947) - Ninomiya Y et al.
Differential expression of two basement membrane collagen genes, COL4A6 and COL4A5, demonstrated by immunofluorescence staining using peptide-specific monoclonal antibodies.
J. Cell Biol. 130: 1219-1229 (1995) (PMID: 7657706) - Naito I et al.
Relationship between COL4A5 gene mutation and distribution of type IV collagen in male X-linked Alport syndrome.
Kidney Int. 50: 304-311 (1996) (PMID: 8807602)
Citations
- Bu L et al.
Somatic Mosaicism in a Male Patient With X-linked Alport Syndrome.
Kidney Int Rep. 14(4): 1031-1035 (2019) (PMID: 31312776) - Samar M et al.
Negative Staining for COL4A5 Correlates With Worse Prognosis and More Severe Ultrastructural Alterations in Males With Alport Syndrome.
Kidney Int Rep. 2(1): 44-52 (2017) (PMID: 29142939) - Malone AF et al.
Functional assessment of a novel COL4A5 splice region variant and immunostaining of plucked hair follicles as an alternative method of diagnosis in X-linked Alport syndrome.
Pediatr Nephrol. 32(6): 997-1003 (2017) (PMID: 28013382) - Nozu K et al.
X-linked Alport syndrome caused by splicing mutations in COL4A5.
Am Soc Nephrol. 9(11): 1958-64 (2014) (PMID: 25183659) - Matsubara S et al.
Pregnancy complicated with Alport syndrome: a good obstetric outcome and failure to diagnose an infant born to a mother with Alport syndrome by umbilical cord immunofluorescence staining.
Obstet Gynaecol Res. 35(6): 1109-14 (2009) (PMID: 20144175) - Patey-Mariaud de Serre N et al.
Collagen alpha5 and alpha2(IV) chain coexpression: analysis of skin biopsies of Alport patients.
Kidney Int. 72(4): 512-6 (2007) (PMID: 17554254) - Kharrat M et al.
Autosomal dominant Alport's syndrome: study of a large Tunisian family.
Kidney Dis Transpl. 17(3): 320-5 (2006) (PMID: 16970251)
