Anti 4-Sulfated Unsaturated Disaccharide Neoepitopes (C-4-S "stubs") of Chondroitin Sulfate or Dermatan Sulfate mAb (Clone 2B6)

Cosmo Bio LTD

Catalog No: CAC-PRPG-BC-M02

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Monoclonal antibody 2B6 recognizes 4-sulfated unsaturated disaccharide neoepitopes (i.e. C-4-S "stubs") generated at the non-reducing terminal of Chondroitin Sulfate or Dermatan Sulfate glycosaminoglycan chains that have been pre-digested with Chondroitinase ABC [see Figure 2; Caterson B (2012) Int. J. Exp. Pathol. 93: 1 - 10] but only Chondroitin Sulfate glycosaminoglycan chains pre-digested with Chondroitinase ACII or only Dermatan Sulfate glycosaminoglycan chains pre-digested with Chondroitinase B.

References:
1) Schwend T, Deaton RJ, Zhang Y, Caterson B & Conrad CW (2012). Corneal sulphated glycosaminoglycans and their effects on trigeminal nerve growth cone behaviour in vitro – roles for ECM in corneal innervation. Invest Opthamol Vis Sci. 53:
2) Caterson B. (2012). Chondroitin sulphate glycosaminoglycans: fun for some and confusion for others. Int. J. of Exp. Path. 93: 1 – 10 PubMed: 22264297
3) Liles M, Palka BP, Harris A,Kerr BC, Hughes CE, Young RD, Meek KM, Caterson B, Quantok AJ (2010). Differential relative sulphation of keratan sulphate glycosaminoglycan in the chick cornea during embryonic development. Invest. Opthalmol. Vis. Sci. 51: 1365-1372 PubMed: 19815728
4) Davies L, Blain E, Caterson B and Duance VC (2008). Chondroitin sulphate impedes the migration of a sub-population of articular cartilage chondrocytes. Osteoarthritis & Cartilage 16: 855 - 864 PubMed: 18222711
5) Hayes AJ, Hughes CE & Caterson B (2008). Antibodies and immunohistochemistry in extracellular matrix research. Methods 45: 10 – 21 PubMed: 18442701
6) Hayes AJ, Hall A, Brown L, Tubo R & Caterson B (2007). Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. J. Histochem. Cytochem. 55: 853 – 866. PubMed: 17478447
7) Caterson B, Mahmoodian F, Sorrell JM, Hardingham TE, Bayliss MT, Carney SL, Ratcliffe A & Muir H (1990). Modulation of native chondroitin sulfate structure in tissue development and in disease. J. Cell Sci. 97: 411 – 417. PubMed: 1705939
8) Mehmet H, Scudder P, Tang, PW, Hounsell, EF, Caterson, B & Feizi T (1986). Antigenic determinants recognized by three monoclonal antibodies to keratan sulfate involve sulfated hepta- or larger oligosaccharides of the poly-N-acetyllactosamine series. Eur. J. Biochem. 157: 385 – 391. PubMed: 2423332
9) Funderburgh JL, Caterson B & Conrad GW (1986). Keratan sulfate proteoglycan during embryonic development of the chicken cornea. Developmental Biology 116: 267 – 277 PubMed: 2942429
10) Katz H, Austen KF, Caterson B, & Stevens RL (1986). Secretory granules of Heparin-containing Rat serosal mast cells also possess highly sulfated chondroitin sulfate proteoglycans. J. Biol. Chem. 261: 13393 - 13396 PubMed: 3531203 
11) Caterson B, Christner JE, Baker JR & Couchman JR (1985). The production and characterization of monoclonal antibodies directed against connective tissue proteoglycans. Federation Proceedings 44: 386 – 393. PubMed: 257841
12) Couchman JR, Caterson B, Christner JE & Baker JR (1984). Mapping by monoclonal antibody detection of glycosaminoglycans in connective tissues. Nature 307: 650 – 652. PubMed: 6420711
13) Caterson B, Christner JE & Baker JR (1983). Identification of a monoclonal antibody that specifically recognizes corneal and skeletal keratan sulfate. Monoclonal antibodies to cartilage proteoglycan. J. Biol. Chem. 258: 8848 – 8854 PubMed: 6223038 8118 – 8137. PubMed: 23132805

Citations for Anti 4-Sulfated Unsaturated Disaccharide Neoepitopes (C-4-S "stubs") of Chondroitin Sulfate or Dermatan Sulfate mAb (Clone 2B6) – 2 Found

Hirota, Yuki; Kubo, Ken-Ichiro; Fujino, Takahiro; Yamamoto, Tokuo T; Nakajima, Kazunori. ApoER2 Controls Not Only Neuronal Migration in the Intermediate Zone But Also Termination of Migration in the Developing Cerebral Cortex. Cerebral Cortex (New York, N.y. : 1991). 2018;28(1):223-235. 

Izumikawa, Tomomi; Dejima, Katsufumi; Watamoto, Yukiko; Nomura, Kazuko H; Kanaki, Nanako; Rikitake, Marika; Tou, Mai; Murata, Daisuke; Yanagita, Eri; Kano, Ai; Mitani, Shohei; Nomura, Kazuya; Kitagawa, Hiroshi. Chondroitin 4-O-Sulfotransferase Is Indispensable for Sulfation of Chondroitin and Plays an Important Role in Maintaining Normal Life Span and Oxidative Stress Responses in Nematodes. The Journal Of Biological Chemistry. 2016;291(44):23294-23304.