Anti CD63 Antigen (LAMP-3/Tspan-30) mAb (Clone 8A12)

Cosmo Bio

SKU:
CAC-SHI-EXO-M02-50
  • Figure 3. IP-WB of human serum exosomes. Sample 1. Anti-CD63 (8A12) - Serum A 2. Control antibody - Serum A 3. Anti-CD63 (8A12) - Serum B 4. Control antibody - Serum B 5. Ultracentrifugation - Serum A 6. Ultracentrifugation - Serum A
  • Figure 4. IP-WB of C32 melanoma cell exosomes. Sample 1. Anti-CD63 (8A12) 2. Control antibody 3. C32 cell exosomes (0.4 ug)
  • Figure 5. Immunofluorescence localization of CD63 in prostate cancer cell PC-3M and colon cancer cell HCT116. A: Prostate cancer cell PC-3MB B: Colon cancer cell HCT116 Secondary antibody: Alexa Fluor® 488-labeled anti-mouse Data provided: Professor Kosuke Kosaka, Department of Molecular Cell Therapy, National Cancer Center Research Institute, Tokyo Medical University Business-Academia Collaboration Course Extracellular vesicle drug discovery research course.
  • Figure 6. CD9 and CD63 molecules on the surface of extracellular vesicles from human breast cancer cell line (MDA-MB-231-luc-D3H2LN)) were detected by anti-human CD9 antibody (SHI-EXO-M01) and anti-human CD63 antibody (SHI-EXO-M02).
  • Figure 7. Comparison of exosome antibodies. (Left) Detection of exosomes with CD9 antibody (clone 12A12) and comparative clone A. Clone A failed to generate a signal while 12A12 detected a strong signal. (Right) Detection of exosomes with CD63 antibody (clone 8A12) and comparative clone A. Clone A detected a weak signal while 8A12 detected a strong signal.
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CD63 (also known as LAMP-3, Melanoma-associated antigen ME491, TSPAN30, MLA1 and OMA81H) is a cell surface glycoprotein which belongs to the tetraspanin superfamily. CD63 is known to complex with integrins. CD63 is expressed on activated platelets, monocytes and macrophages and is weakly expressed on granulocytes, T cells and B cells. Importantly, it is found on the surface of exosomes.

Exosomes are cell-derived vesicles bounded by a lipid bilayer membrane and exhibiting a diameter of 50 to 150 nm. They are secreted from cultured cells and are observed in body fluids such as saliva, blood, urine, amniotic fluid, malignant ascites. Recent studies indicate that exosomes contain various proteins and RNAs, suggesting a role in information transfer between cells.

This monoclonal antibody can be used to immunoprecipitate exosomes from serum and culture supernatants.

References:
1) Yoshioka Y et al., Nat Commun. 2014 Apr 7;5:3591. doi: 10.1038/ncomms4591.
2) N Nishida-Aoki et al., Mol Ther. 2017 Jan 4;25(1):181-191. doi: 10.1016/j.ymthe.2016.10.009.
3) Saito S et al., Sci Rep. 2018 Mar 5;8(1):3997. doi: 10.1038/s41598-018-22450-2.



Product Specifications
Application IP, WB, IF, FC
Reactivity Human
Clonality Monoclonal (Clone No.: 8A12)
Host Mouse


Documents & Links for Anti CD63 Antigen (LAMP-3/Tspan-30) mAb (Clone 8A12)
Datasheet Anti CD63 Antigen (LAMP-3/Tspan-30) mAb (Clone 8A12)

Documents & Links for Anti CD63 Antigen (LAMP-3/Tspan-30) mAb (Clone 8A12)
Datasheet Anti CD63 Antigen (LAMP-3/Tspan-30) mAb (Clone 8A12)



Citations for Anti CD63 Antigen (LAMP-3/Tspan-30) mAb (Clone 8A12) – 2 Found
Jingushi, Kentaro; Uemura, Motohide; Nakano, Kosuke; Hayashi, Yujiro; Wang, Cong; Ishizuya, Yu; Yamamoto, Yoshiyuki; Hayashi, Takuji; Kinouchi, Toshiro; Matsuzaki, Kyosuke; Kato, Taigo; Kawashima, Atsunari; Ujike, Takeshi; Nagahara, Akira; Fujita, Kazutoshi; Ueda, Koji; Tsujikawa, Kazutake; Nonomura, Norio. Leukocyte‑associated immunoglobulin‑like receptor 1 promotes tumorigenesis in RCC. Oncology Reports. 2019;41(2):1293-1303.  PubMed
Matsumura, Sachiko; Minamisawa, Tamiko; Suga, Kanako; Kishita, Hiromi; Akagi, Takanori; Ichiki, Takanori; Ichikawa, Yuki; Shiba, Kiyotaka. Subtypes of tumour cell-derived small extracellular vesicles having differently externalized phosphatidylserine. Journal Of Extracellular Vesicles. 8(1):1579541.  PubMed
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