Anti CD44 Antigen v9 mAb (Clone RV3)

Cosmo Bio LTD

Catalog No: CAC-LKG-M003

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Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.

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CD44 is a single-pass type I transmembrane protein and functions as a cellular adhesion molecule for hyaluronic acid, a major component of the extracellular matrix. It exists in numerous isoforms that are generated through alternative splicing of CD44 precursor mRNA. Whereas the standard isoform of CD44 (CD44s) is expressed predominantly in hematopoietic cells and normal epithelial cell subsets, CD44v (variant) isoforms, which contain additional insertions in the membrane-proximal extracellular region, are highly expressed in epithelial-type carcinomas. Moreover, CD44 is reported to be a cell surface marker for cancer stem cells (CSCs) derived from solid tumors including breast, prostate, colon, head and neck and pancreatic cancer. Expression of CD44, especially variant isoforms (CD44 v8-10), contributes to reactive oxygen species (ROS) defense through upregulation of the synthesis of reduced glutathione (GSH), the primary intracellular antioxidant. CD44 v8-10 interacts with and stabilizes xCT, a subunit of the cystine-glutamate transporter xc(-), and thereby promotes cystine uptake for GSH synthesis. The ability to avoid the consequences of exposure to high levels of ROS is required for cancer cell survival and propagation in vivo. CSCs (whose defense against ROS is enhanced by CD44v8-10) are thus thought to drive tumor growth, chemoresistance and metastasis. Clone RV3 (a monoclonal antibody specific for human CD44 v9) can be used in flow cytometry, and importantly, for the enrichment of CSCs using FACS. RV3 can be applied towards understanding a variety of molecular mechanisms and towards the development of new medicines against cancer stem cells using in vitro cell-based assays such as the "in vitro sphere formation" and "in vivo lung metastasis" assays.

References:
1) Nagano O., et al., Oncogene. 2013 Jan 21., 1-8. PMID:23334333
2) Ishimoto T., et al., Cancer Cell. 2011 Mar 8;19(3):387-400. PMID : 21397861
3) Yae T., et al., Nat Commun. 2012 Jun 6;3:883. PMID: 22673910
4) Tsugawa H., et al., Cell Host Microbe. 2012 Dec 13;12(6):764-77. PMID: 23245321
5) Tanabe KK., et al., Lancet. 1993 Mar 20;341(8847):725-6. PMID: 8095628

Product Specifications

• Application: FC, IP, ELISA, IF, IHC(p), WB, ICC
• Reactivity: Human
• Clonality: Monoclonal (Clone No.: RV3)
• Host: Rat

Citations for Anti CD44 Antigen v9 mAb (Clone RV3) – 35 Found

Jeong, Sangho; Choi, Eunyoung; Petersen, Christine P; Roland, Joseph T; Federico, Alessandro; Ippolito, Rossana; D'Armiento, Francesco P; Nardone, Gerardo; Nagano, Osamu; Saya, Hideyuki; Romano, Marco; Goldenring, James R. Distinct metaplastic and inflammatory phenotypes in autoimmune and adenocarcinoma-associated chronic atrophic gastritis. United European Gastroenterology Journal. 2017;5(1):37-44. 

Nagano, O; Okazaki, S; Saya, H. Redox regulation in stem-like cancer cells by CD44 variant isoforms. Oncogene. 2013;32(44):5191-8. 

Ogihara, Koichiro; Kikuchi, Eiji; Okazaki, Shogo; Hagiwara, Masayuki; Takeda, Toshikazu; Matsumoto, Kazuhiro; Kosaka, Takeo; Mikami, Shuji; Saya, Hideyuki; Oya, Mototsugu. Sulfasalazine could modulate the CD44v9-xCT system and enhance cisplatin-induced cytotoxic effects in metastatic bladder cancer. Cancer Science. 2019;110(4):1431-1441. 

Takeda, Mitsunobu; Koseki, Jun; Takahashi, Hidekazu; Miyoshi, Norikatsu; Nishida, Naohiro; Nishimura, Junichi; Hata, Taishi; Matsuda, Chu; Mizushima, Tsunekazu; Yamamoto, Hirofumi; Ishii, Hideshi; Doki, Yuichiro; Mori, Masaki; Haraguchi, Naotsugu. Disruption of Endolysosomal RAB5/7 Efficiently Eliminates Colorectal Cancer Stem Cells. Cancer Research. 2019;79(7):1426-1437. 

Osaki, Luciana H; Bockerstett, Kevin A; Wong, Chun F; Ford, Eric L; Madison, Blair B; DiPaolo, Richard J; Mills, Jason C. Interferon-γ directly induces gastric epithelial cell death and is required for progression to metaplasia. The Journal Of Pathology. 2019;247(4):513-523. 

Akamine, Takaki; Tagawa, Tetsuzo; Ijichi, Kayo; Toyokawa, Gouji; Takamori, Shinkichi; Hirai, Fumihiko; Okamoto, Tatsuro; Oda, Yoshinao; Maehara, Yoshihiko. The Significance of CD44 Variant 9 in Resected Lung Adenocarcinoma: Correlation with Pathological Early-Stage and EGFR Mutation. Annals Of Surgical Oncology. 2019;26(5):1544-1551. 

Tokunaga, Eriko; Fujita, Aya; Takizawa, Katsumi; Baba, Kimiko; Akiyoshi, Sayuri; Nakamura, Yoshiaki; Ijichi, Hideki; Masuda, Takanobu; Koga, Chinami; Tajiri, Wakako; Ohno, Shinji; Taguchi, Kenichi; Ishida, Mayumi. CD44v9 as a poor prognostic factor of triple-negative breast cancer treated with neoadjuvant chemotherapy. Breast Cancer (Tokyo, Japan). 2019;26(1):47-57. 

Aguilar-Chaparro, Mario Alejandro; Rivera-Pineda, Sonia Andrea; Hernández-Galdámez, Hury Viridiana; Piña-Vázquez, Carolina; Villa-Treviño, Saúl. The CD44std and CD44v9 subpopulations in non-tumorigenic invasive SNU-423 cells present different features of cancer stem cells. Stem Cell Research. 2023;72( 37844417):103222. 

Fontanella, Rosaria A; Sideri, Silvia; Di Stefano, Chiara; Catizone, Angiolina; Di Agostino, Silvia; Angelini, Daniela F; Guerrera, Gisella; Battistini, Luca; Battafarano, Giulia; Del Fattore, Andrea; Campese, Antonio Francesco; Padula, Fabrizio; De Cesaris, Paola; Filippini, Antonio; Riccioli, Anna. CD44v8-10 is a marker for malignant traits and a potential driver of bone metastasis in a subpopulation of prostate cancer cells. Cancer Biology & Medicine. 2021;18(3):788-807. 

Fontanella, Rosaria A; Sideri, Silvia; Di Stefano, Chiara; Catizone, Angiolina; Di Agostino, Silvia; Angelini, Daniela F; Guerrera, Gisella; Battistini, Luca; Battafarano, Giulia; Del Fattore, Andrea; Campese, Antonio Francesco; Padula, Fabrizio; De Cesaris, Paola; Filippini, Antonio; Riccioli, Anna. CD44v8-10 is a marker for malignant traits and a potential driver of bone metastasis in a subpopulation of prostate cancer cells. Cancer Biology & Medicine. 2021;18(3):788-807. 

Jogo, Tomoko; Oki, Eiji; Nakanishi, Ryota; Ando, Koji; Nakashima, Yuichiro; Kimura, Yasue; Saeki, Hiroshi; Oda, Yoshinao; Maehara, Yoshihiko; Mori, Masaki. Expression of CD44 variant 9 induces chemoresistance of gastric cancer by controlling intracellular reactive oxygen spices accumulation. Gastric Cancer : Official Journal Of The International Gastric Cancer Association And The Japanese Gastric Cancer Association. 2021;24(5):1089-1099. 

Thanee, Malinee; Padthaisong, Sureerat; Suksawat, Manida; Dokduang, Hasaya; Phetcharaburanin, Jutarop; Klanrit, Poramate; Titapun, Attapol; Namwat, Nisana; Wangwiwatsin, Arporn; Sa-Ngiamwibool, Prakasit; Khuntikeo, Narong; Saya, Hideyuki; Loilome, Watcharin. Sulfasalazine modifies metabolic profiles and enhances cisplatin chemosensitivity on cholangiocarcinoma cells in in vitro and in vivo models. Cancer & Metabolism. 2021;9(1):11. 

Thanee, Malinee; Padthaisong, Sureerat; Suksawat, Manida; Dokduang, Hasaya; Phetcharaburanin, Jutarop; Klanrit, Poramate; Titapun, Attapol; Namwat, Nisana; Wangwiwatsin, Arporn; Sa-Ngiamwibool, Prakasit; Khuntikeo, Narong; Saya, Hideyuki; Loilome, Watcharin. Sulfasalazine modifies metabolic profiles and enhances cisplatin chemosensitivity on cholangiocarcinoma cells in in vitro and in vivo models. Cancer & Metabolism. 2021;9(1):11. 

Padthaisong, Sureerat; Thanee, Malinee; Namwat, Nisana; Phetcharaburanin, Jutarop; Klanrit, Poramate; Khuntikeo, Narong; Titapun, Attapol; Sungkhamanon, Sakkarn; Saya, Hideyuki; Loilome, Watcharin. Overexpression of a panel of cancer stem cell markers enhances the predictive capability of the progression and recurrence in the early stage cholangiocarcinoma. Journal Of Translational Medicine. 2020;18(1):64. 

Padthaisong, Sureerat; Thanee, Malinee; Namwat, Nisana; Phetcharaburanin, Jutarop; Klanrit, Poramate; Khuntikeo, Narong; Titapun, Attapol; Sungkhamanon, Sakkarn; Saya, Hideyuki; Loilome, Watcharin. Overexpression of a panel of cancer stem cell markers enhances the predictive capability of the progression and recurrence in the early stage cholangiocarcinoma. Journal Of Translational Medicine. 2020;18(1):64. 

Fujimoto, Takuya; Tsunedomi, Ryouichi; Matsukuma, Satoshi; Yoshimura, Kiyoshi; Oga, Atsunori; Fujiwara, Nobuyuki; Fujiwara, Yasuhiro; Matsui, Hiroto; Shindo, Yoshitaro; Tokumitsu, Yukio; Suzuki, Nobuaki; Kobayashi, Shogo; Hazama, Shoichi; Eguchi, Hidetoshi; Nagano, Hiroaki. Cathepsin B is highly expressed in pancreatic cancer stem-like cells and is associated with patients' surgical outcomes. Oncology Letters. 2021;21(1):30. 

Malinee Thanee1 Sureerat Padthaisong2 Manida Suksawat2 Hasaya Dokduang3 Jutarop Phetcharaburanin4 Poramate Klanrit4 Attapol Titapun5 Nisana Namwat4 Prakasit Sa- ngiamwibool6 Narong Khuntikeo5 Hideyuki Saya7 Watcharin Loilome8 . Sulfasalazine Modi es Metabolic Pro les and Enhances Cisplatin Chemosensitivity on Cholangiocarcinoma Cells in in vitro and in vivo models. 2020; 
- Comments: https://web.archive.org/web/20200603054649id_/https://assets.researchsquare.com/files/rs-31522/v1/5b46db96-888d-4301-8e0a-2e64eaa0ce81.pdf

Ueda, Shinobu; Takanashi, Masakatsu; Sudo, Katsuko; Kanekura, Kohsuke; Kuroda, Masahiko. miR-27a ameliorates chemoresistance of breast cancer cells by disruption of reactive oxygen species homeostasis and impairment of autophagy. Laboratory Investigation; A Journal Of Technical Methods And Pathology. 2020;100(6):863-873. 

Goto, Nohara; Suzuki, Hiroyuki; Zheng, Ling; Okano, Yasuhito; Okita, Yukari; Watanabe, Yukihide; Kato, Yukinari; Kato, Mitsuyasu. Promotion of squamous cell carcinoma tumorigenesis by oncogene-mediated THG-1/TSC22D4 phosphorylation. Cancer Science. 2023;114(10):3972-3983. 

Takizawa, Kohei; Muramatsu, Koji; Maruyama, Kouji; Urakami, Kenichi; Sugino, Takashi; Kusuhara, Masatoshi; Yamaguchi, Ken; Ono, Hiroyuki; Kitagawa, Yuko. Metabolic Profiling of Human Gastric Cancer Cells Treated With Salazosulfapyridine. Technology In Cancer Research & Treatment. 2020;19:1533033820928621. 

Trinh, Vincent Quoc-Huy; Ankenbauer, Katherine E; Liu, Jiayue; Batardiere, Maelle; Maurer, H Carlo; Copeland, Celina; Wong, Jahg; Ben-Levy, Olivia; Torbit, Sabrina M; Jarvis, Brenda; Revetta, Frank; Ivanov, Sergey; Jyotsana, Nidhi; Makino, Yuki; Ruelas, Amanda M; Means, Anna L; Maitra, Anirban; Tan, Marcus C B; DelGiorno, Kathleen E. Oncogenic GNAS drives a gastric pylorus program in intraductal papillary mucinous neoplasms of the pancreas. Biorxiv : The Preprint Server For Biology. 2024; 38464029 

Titapun, Attapol; Luvira, Vor; Srisuk, Tharatip; Jareanrat, Apiwat; Thanasukarn, Vasin; Thanee, Malinee; Sa-Ngiamwibool, Prakasit; Padthaisong, Sureerat; Duangkumpha, Kassaporn; Suksawat, Manida; Loilome, Watcharin; Sithithaworn, Paiboon; Techasen, Anchalee; Thinkhamrop, Bandit; Dzienny, Alexa; Caglayan, Ayse; Park, David; Mahmud, Simran; Khuntikeo, Narong. High Levels of Serum IgG for Opisthorchis viverrini and CD44 Expression Predict Worse Prognosis for Cholangiocarcinoma Patients after Curative Resection. International Journal Of General Medicine. 14:2191-2204. 

Druffner, Sara R; Venkateshwaraprabu, Shrinidhi; Khadka, Stuti; Duncan, Benjamin C; Morris, Maeve T; Sen-Kilic, Emel; Damron, Fredrick H; Liechti, George W; Busada, Jonathan T. Comparison of gastric inflammation and metaplasia induced by Helicobacter pylori or Helicobacter felis colonization in mice. Microbiology Spectrum. 2024;12(6):e0001524. 

MONTELEONE, LORENZO. Role of PKC-α in the induction of ferroptosis: a therapeutic target to fight chemoresistance of cancer stem cells. 2022; 
- Comments: https://tesidottorato.depositolegale.it/handle/20.500.14242/70452

Monteleone, Lorenzo; Speciale, Andrea; Valenti, Giulia Elda; Traverso, Nicola; Ravera, Silvia; Garbarino, Ombretta; Leardi, Riccardo; Farinini, Emanuele; Roveri, Antonella; Ursini, Fulvio; Cantoni, Claudia; Pronzato, Maria Adelaide; Marinari, Umberto Maria; Marengo, Barbara; Domenicotti, Cinzia. PKCα Inhibition as a Strategy to Sensitize Neuroblastoma Stem Cells to Etoposide by Stimulating Ferroptosis. Antioxidants (Basel, Switzerland). 2021;10(5) 

Jeong, Haengdueng; Lee, Buhyun; Kim, Kwang H; Cho, Soo Young; Cho, Yejin; Park, Jeongeun; Lee, Yura; Oh, Yeseul; Hwang, Bo Ram; Jang, Ah-Ra; Park, Jong-Hwan; Park, Ji-Ho; Jeong, Sang-Ho; Lee, Daekee; Lee, Yong Chan; Lim, Kyung-Min; Goldenring, James R; Nam, Ki Taek. WFDC2 Promotes Spasmolytic Polypeptide-Expressing Metaplasia Through the Up-Regulation of IL33 in Response to Injury. Gastroenterology. 2021;161(3):953-967.e15. 

Krishnan, Vaidehi; Lim, Debbie Xiu En; Hoang, Phuong Mai; Srivastava, Supriya; Matsuo, Junichi; Huang, Kie Kyon; Zhu, Feng; Ho, Khek Yu; So, Jimmy Bok Yan; Khor, Christopher; Tsao, Stephen; Teh, Ming; Fock, Kwong Ming; Ang, Tiing Leong; Jeyasekharan, Anand D; Tan, Patrick; Yeoh, Khay-Guan; Ito, Yoshiaki. DNA damage signalling as an anti-cancer barrier in gastric intestinal metaplasia. Gut. 2020;69(10):1738-1749. 

Yoshikawa, Momoko; Tsuchihashi, Kenji; Ishimoto, Takatsugu; Yae, Toshifumi; Motohara, Takeshi; Sugihara, Eiji; Onishi, Nobuyuki; Masuko, Takashi; Yoshizawa, Kunio; Kawashiri, Shuichi; Mukai, Makio; Asoda, Seiji; Kawana, Hiromasa; Nakagawa, Taneaki; Saya, Hideyuki; Nagano, Osamu. xCT inhibition depletes CD44v-expressing tumor cells that are resistant to EGFR-targeted therapy in head and neck squamous cell carcinoma. Cancer Research. 2013;73(6):1855-66. 

Senbanjo, Linda T; AlJohani, Hanan; Majumdar, Sunipa; Chellaiah, Meenakshi A. Characterization of CD44 intracellular domain interaction with RUNX2 in PC3 human prostate cancer cells. Cell Communication And Signaling : Ccs. 2019;17(1):80. 

Ogihara, Koichiro; Kikuchi, Eiji; Okazaki, Shogo; Hagiwara, Masayuki; Takeda, Toshikazu; Matsumoto, Kazuhiro; Kosaka, Takeo; Mikami, Shuji; Saya, Hideyuki; Oya, Mototsugu. Sulfasalazine could modulate the CD44v9-xCT system and enhance cisplatin-induced cytotoxic effects in metastatic bladder cancer. Cancer Science. 2019;110(4):1431-1441. 

O Nagano, S Okazaki & H Saya . Redox regulation in stem-like cancer cells by CD44 variant isoforms. Oncogene. 2013;32(44):5191-8. 
- Comments: https://www.nature.com/articles/onc2012638.pdf

Junko Ohtsuka1,2, Hiroko Oshima3, Issei Ezawa1,4, Ryo Abe2, Masanobu Oshima3 & Rieko Ohki1. Functional loss of p53 cooperates with the in vivo microenvironment to promote malignant progression of gastric cancers. Sci Rep. 2018;8(1):2291. 

Prominent role of RAB39A-RXRB axis in cancer development and stemness. Oncotarget. 2018;9(11):9852-9866. 

Nishino, Makoto; Ozaki, Mari; Hegab, Ahmed E; Hamamoto, Junko; Kagawa, Shizuko; Arai, Daisuke; Yasuda, Hiroyuki; Naoki, Katsuhiko; Soejima, Kenzo; Saya, Hideyuki; Betsuyaku, Tomoko. Variant CD44 expression is enriching for a cell population with cancer stem cell-like characteristics in human lung adenocarcinoma. Journal Of Cancer. 8(10):1774-1785. 

Choi, Eun-Seok; Kim, Hyunjin; Kim, Hyung-Pyo; Choi, Yongdoo; Goh, Sung-Ho. CD44v8-10 as a potential theranostic biomarker for targeting disseminated cancer cells in advanced gastric cancer. Scientific Reports. 2017;7(1):4930.