c-Myc is a very potent oncogene upregulated in various types of cancer. c-Myc, through its role as a transcription factor, alters the expression of hundreds of target genes, often oncogenes or tumor suppressors. Clone 9E10 recognizes recombinant proteins that incorporate the c-Myc epitope tag and detects human c-Myc proteins and peptides in random coil rather than alpha-helix structures.
![Example Assay Data:
1. Sample Preparation.
Inserted c-Myc tag to C-terminus end of DasherGFP plasmid vector (DNA2.0)
Protein was expressed in E.coli and lysed (Crude sample).
Crude sample as separated by SDS-PAGE. Part of the fraction was used for CBB Staining, rest for Western Blotting.
2. Immunoblotting analysis.
Primary Antibody (anti c-Myc [CAC-XIM-MA001]) 1 : 1000 dilution (1μg/mL)
Secondary Antibody (anti mouse IgG-HRP, Bethyl Laboratories, Cat.No. A90-216P) 1 : 3000 dilution
Anti c-Myc [CAC-XIM-MA001] detection of c-Myc tag:
Based on CBB Staining result, the c-Myc tagged fluorescent protein exhibited a higher molecular weight compared to the non-tagged version of fluorescent protein. Further, the Anti c-Myc antibody detected the c-Myc tagged- but not the non-tagged version of fluorescent protein.](https://cdn11.bigcommerce.com/s-ydswqc5qsc/images/stencil/500x659/products/29850/120401/cac-xim-ma001_anti-c-myc-mab-clone-9e10_7785__14705.1745620901.png?c=2 "Example Assay Data:
1. Sample Preparation.
Inserted c-Myc tag to C-terminus end of DasherGFP plasmid vector (DNA2.0)
Protein was expressed in E.coli and lysed (Crude sample).
Crude sample as separated by SDS-PAGE. Part of the fraction was used for CBB Staining, rest for Western Blotting.
2. Immunoblotting analysis.
Primary Antibody (anti c-Myc [CAC-XIM-MA001]) 1 : 1000 dilution (1μg/mL)
Secondary Antibody (anti mouse IgG-HRP, Bethyl Laboratories, Cat.No. A90-216P) 1 : 3000 dilution
Anti c-Myc [CAC-XIM-MA001] detection of c-Myc tag:
Based on CBB Staining result, the c-Myc tagged fluorescent protein exhibited a higher molecular weight compared to the non-tagged version of fluorescent protein. Further, the Anti c-Myc antibody detected the c-Myc tagged- but not the non-tagged version of fluorescent protein.")