Anti Auxin Inducible Degron (AID/IAA17) Tag mAb

Cosmo Bio

SKU: CAC-APC004AM-T

Background

Detection of intracellular AID plasmid (pAID1.1-N Vector, pAID1.1-C Vector or Luc-AID NLS plasmid) expression is very critical for scientists who integrate them into cells. Anti-AID protein tag antibody (IAA17) provides an excellent means for monitoring gene expression and protein localization in living cells. Monoclonal anti-AID tag (IAA17) antibody can detect AID tag (IAA17) in immunoblot experiments.

Application

This antibody reacts with IAA17 on Western blotting.
This antibody also detects AID tag conjugated with protein expressed in mammalian cells and Yeast cells on Western blotting.

WB : 1 : 500 - 1 : 2,000
Other applications have not been tested

Feature and Advantages

The mouse was immunized with recombinant AID tag (IAA17) protein.
This antibody was purified from mouse using Purified IgG
This antibody solution contains PBS, and 0.1% sodium azide as preservative.
*Azide may react with copper or lead in plumbing system to form explosive metal azides. Please always flush plenty of water when disposing materials containing azide to drain.

References

Nature Methods (2009) 6:917-923

Product Specifications

• Application: WB
• Clonality: Monoclonal
• Host: Mouse

Citations for Anti Auxin Inducible Degron (AID/IAA17) Tag mAb – 3 Found

Pakchuen, Sujiraporn; Ishibashi, Mai; Takakusagi, Emi; Shirahige, Katsuhiko; Sutani, Takashi. Physical Association of Saccharomyces cerevisiae Polo-like Kinase Cdc5 with Chromosomal Cohesin Facilitates DNA Damage Response. The Journal Of Biological Chemistry. 2016;291(33):17228-46. 

Verzijlbergen, Kitty F; Nerusheva, Olga O; Kelly, David; Kerr, Alastair; Clift, Dean; de Lima Alves, Flavia; Rappsilber, Juri; Marston, Adele L. Shugoshin biases chromosomes for biorientation through condensin recruitment to the pericentromere. Elife. 3( 24497542):e01374. 

Guérin, Thomas M; Barrington, Christopher; Pobegalov, Georgii; Molodtsov, Maxim I; Uhlmann, Frank. An extrinsic motor directs chromatin loop formation by cohesin. The Embo Journal. 2024; 39160275